Auxin
Tocris Bioscience, part of Bio-Techne | Catalog # 6834
Chemical dimerizer used in auxin-inducible degron (AID) system; phytohormone
Key Product Details
Description
Chemical dimerizer used in auxin-inducible degron (AID) system; phytohormone
Product Description
Auxin is a chemical dimerizer used in auxin-inducible degron (AID) systems. Induces degradation of a target protein tagged with the auxin-receptor F-box protein Tir1 E3 ligase AID in human colorectral cancer and mouse ES cells or tagged with AFB2 in A431 cells. Endogenous plant hormone.Product Specifications
Molecular Weight
175.18
Formula
C10H9NO2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
1H-Indole-3-acetic acid
CAS Number
87-51-4
PubChem ID
802
InChI Key
SEOVTRFCIGRIMH-UHFFFAOYSA-N
SMILES
OC(CC1=CNC2=C1C=CC=C2)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 17.52 | 100 | |
| Ethanol | 17.52 | 100 |
Preparing Stock Solutions
for Auxin
The following data is based on the product molecular weight 175.18
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.71 mL | 28.54 mL | 57.08 mL |
| 5 mM | 1.14 mL | 5.71 mL | 11.42 mL |
| 10 mM | 0.57 mL | 2.85 mL | 5.71 mL |
| 50 mM | 0.11 mL | 0.57 mL | 1.14 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Natsume Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 2016 PMID: 27052166
- Li An efficient auxin-inducible degron system with low basal degradation in human cells. Nat.Methods. 2019 PMID: 31451765
- Nishimura An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat.Methods 2009 PMID: 19915560
Product Specific Notices for Auxin
For research use only
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