Ac-Cys-NHMe

Product Description

Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery.

Solubility

Preparing Stock Solutions for Ac-Cys-NHMe

The following data is based on the product molecular weight 176.23

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.67 mL	28.37 mL	56.74 mL
5 mM	1.13 mL	5.67 mL	11.35 mL
10 mM	0.57 mL	2.84 mL	5.67 mL
50 mM	0.11 mL	0.57 mL	1.13 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Hofmann Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins. Nat.Chem. 2020 PMID: 32929246
• Levasseur Post-assembly modification of protein cages by Ubc9-mediated lysine acylation. Chembiochem 2022 PMID: 35951442