Mouse Methylcellulose Complete Media for Pre-B Cells

For the growth and enumeration of mouse precursor B (pre-B) cells, optimized with IL-7.

Why use R&D Systems Mouse Methylcellulose Complete Media for Pre-B Cells for Colony Forming Cell Assays?

Colony forming cell (CFC) assays, which are used to enumerate and quantify multi-potent and single lineage hematopoietic progenitors, can be time consuming and laborious.

Successful growth and enumeration of cell colonies is dependent on factors such as accurate cell counts, the presence of growth factors and/or cytokines, adequate humidity, and the use of high quality media. R&D Systems offers Mouse Methylcellulose Complete Media for Pre-B Cells which contains Recombinant Mouse IL-7, a cytokine that promotes proliferation of pre- and pro-B cells of mouse origin. Pre-B cells are derived from progenitor B (pro-B) cells and mature into immature B cells in response to cytokines. In addition to their role in normal B cell development, pre-B and immature B cells are associated with certain leukemias.

R&D Systems Mouse Methylcellulose Complete Media for Pre-B cells:

• Optical clarity facilitates colony identification.
• High lot-to-lot consistency decreases variation.
• Supports reproducible in vitro growth of pre-B progenitor cells of mouse origin.
• Does not require addition of growth factors or cytokines.
• Increased cloning efficiency and improved colony growth compared to agar.

• 100 mL of Mouse Methylcellulose Complete Media for Pre-B Cells.

Stability and Storage

Mouse Methylcellulose Complete Media for Pre-B Cells should be stored at ≤-20 °C upon receipt. Storage at 2 °C to 8 °C is not recommended.

Precaution

• The acute and chronic effects of overexposure to this media are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media.

Limitations

• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• The reagent should not be used beyond the expiration date indicated on the vial labels.
• The media is optimized to assay mouse hematopoietic progenitors and is ineffective with human hematopoietic progenitors.
• Derivation of mouse hematopoietic progenitors from different individual animals may cause results to vary.

Mouse Pre-B Colony Formation. Mouse Methylcellulose Complete Media for Pre-B Cells (Catalog # HSC009) was used in the colony forming assay to support growth of pre-B progenitor cells of mouse origin. Both images show colony forming unit Pre-B colonies at 40X magnification. Each individual colony consists of a homogeneous population of tiny, round or oval shaped cells. CFU Pre-B colonies vary in size and morphology. Some colonies form a tight and compact spheroid while others are diffuse colonies with cells in the periphery.

Mouse Methylcellulose Stock and Base Media

Complete Mouse Methylcellulose Media

*Base media and stock solutions do not contain cytokines and will not support colony growth unless conditioned media, cytokines, or other culture supplements are added.

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte­nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu­ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple­tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.