Human Methylcellulose Serum-Free Enriched Media Without Epo

Kit Summary

Complete, defined media for the differentiation and enumeration of myeloid progenitor cells and for the detection of Epo-independent erythropoiesis.

Why use R&D Systems Human Methylcellulose Serum-Free Enriched Media Without Epo for Colony Forming Cell Assays?

Colony forming cell (CFC) assays, which are used to enumerate and quantify multi-potent and single lineage hematopoietic progenitors, can be time consuming and laborious.

Successful growth and enumeration of cell colonies is dependent on factors such as accurate cell counts, the presence of growth factors and/or cytokines, adequate humidity, and the use of high quality media. Additionally, experimental variation can be reduced by using defined medium. R&D Systems offers Human Methylcellulose Serum-Free Enriched Media Without Epo, a define medium which contains all of the growth factors and cytokines needed to support optimal colony growth and enumeration. The Human Methylcellulose Serum-Free Enriched Media Without Epo is specially formulated and has been optimized for CFC assays detecting colony-forming myeloid progenitors (CFU-GM, CFU-G, CFU-M) in human bone marrow, cord blood, mobilized peripheral blood, and peripheral blood samples. It can also be used in CFC assays for purified human hematopoietic progenitor cells. This product is recommended for use in the CFC assays, when a serum-Free defined condition is desired.

R&D Systems Human Methylcellulose Serum-Free Enriched Media Without Epo:

• Optical clarity facilitates colony identification.
• Suitable for detection of Epo-independent erythropoiesis.
• High lot-to-lot consistency decreases variation.
• Supports reproducible in vitro growth of myeloid progenitor cells.
• Defined media reduces experimental variation.
• Increased cloning efficiency and improved colony growth compared to agar.

Kit Contents

• 100 mL of Human Methylcellulose Serum-Free Enriched Media Without Epo.

Stability and Storage

Human Methylcellulose Serum-Free Enriched Media Without Epo should be stored at ≤-20 °C upon receipt. Storage at 2 °C to 8 °C is not recommended.

Precautions

• The acute and chronic effects of overexposure to this media are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media.
• The human Transferrin used in this product was derived from human plasma, which has been tested and found negative for HIV-1/2 antibodies, Hepatitis B surface antigen, Hepatitis C antibody, Syphilis, and p24 antigen by FDA approved methods. Handle as if capable of transmitting infection, and dispose of according to applicable regulations.

Limitations

• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• The reagent should not be used beyond the expiration date indicated on the vial labels.
• The media is optimized to assay human hematopoietic progenitors and is ineffective with mouse hematopoietic progenitors.
• Derivation of human hematopoietic progenitors from different individuals may cause results to vary.

Data Examples

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Human Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. A. Colony forming unit-granulocyte (CFU-G) are clonogenic progenitors of granulocytes that give rise to a homogeneous population of eosinophils, basophils, or neutrophils. B. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. C. Colony forming unit-macrophage (CFU-M) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages.

Guide to Choosing Media for the Colony Forming Cell (CFC) Assay

Human Methylcellulose Stock and Base Media

Complete Human Methylcellulose Media

*Base media and stock solutions do not contain cytokines and will not support colony growth unless conditioned media, cytokines, or other culture supplements are added.