Ocean Blue, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 6489
Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Key Product Details
Description
Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Product Description
Key information: Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.Application: Flow cytometry, fluorescence microscopy.
Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M-1cm-1; A280 correction factor = 0.15.
Please see the protocol for further information on protein/antibody labeling and conjugation.
Note: This product is typically prepared in DMSO.
Optical Data
| Emission Color | Blue |
| Brightness | |
| λabs | 405 nm |
| λem | 455 nm |
| Extinction Coefficient (ε) | 36000 M-1cm-1 |
| Quantum Yield (φ) | 0.78 |
| Closest Laser line | 405 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.15 |
| Application | Flow Cytometry, Fluorescence Microscopy |
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Product Specifications
Molecular Weight
339.20
Formula
C14H7F2NO7
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
CAS Number
215868-33-0
PubChem ID
56927770
InChI Key
NZYXABPVYJRICY-UHFFFAOYSA-N
SMILES
OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Cox Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 2004 PMID: 14740493
- Brandtzaeg The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 1998 PMID: 9760215
- Haugland Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. 2001 PMID: 18228336
- Sun Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 1998 PMID: 9873685
Product Specific Notices for Ocean Blue, SE
For research use only
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