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VECTASHIELD(R) Antifade Mounting Medium with DAPI

Novus Biologicals, part of Bio-Techne | Catalog # H-1200-NB

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H-1200-NB

Key Product Details

Applications

Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Microscopy

Product Summary for VECTASHIELD(R) Antifade Mounting Medium with DAPI

VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Non-hardening formulation
  • No counterstain


  • The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4C, protected from light.

    VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.

    The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 degrees C, protected from light. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.

    Both the VECTASHIELD HardSet and the original VECTASHIELD Mounting Media are available with or without the counterstain DAPI (4,6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

    Product Specifications

    Application Notes

    The refractive index for VECTASHIELD Mounting Medium is 1.45. Use in IHC-Fr reported in scientific publication (PMID: 32452384)..

    Scientific Data Images for VECTASHIELD(R) Antifade Mounting Medium with DAPI

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.
    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.
    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB]

    In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.

    Formulation, Preparation, and Storage

    Concentration

    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C in the dark.

    Product Documents for VECTASHIELD(R) Antifade Mounting Medium with DAPI

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for VECTASHIELD(R) Antifade Mounting Medium with DAPI

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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