Methylene Blue

Name: Methylene Blue
Brand: Tocris Bioscience
SKU: 3213
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3213

(1)

COA / SDS

Biological stain; also neuroprotective and antimalarial

Discontinued Product

3213 has been discontinued. View all Histology Stains products.

Key Product Details

Description:	Biological stain; also neuroprotective and antimalarial
Chemical Name:	3,7-bis(Dimethylamino)phenazathionium chloride
Molecular Weight:	319.85

View Full Technical Details for Methylene Blue

Technical Data
Citations / Reviews (1)

Biological Activity for Methylene Blue

Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC₅₀ values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC₅₀ = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.

Technical Data for Methylene Blue

M. Wt	319.85
Formula	C₁₆H₁₈ClN₃S
Storage	Store at RT
CAS Number	61-73-4
PubChem ID	6099
InChi Key	CXKWCBBOMKCUKX-UHFFFAOYSA-M
Smiles	CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Methylene Blue

References are publications that support the biological activity of the product.

Poteet Neuroprotective actions of methylene blue and its derivatives. PLoS One 2012 PMID: 23118969
Wen Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. J.Biol.Chem. 2011 PMID: 21454572
Mayer Inhibition of nitric oxide synthesis by methylene blue. Biochem.Pharmacol. 1993 PMID: 7679577
Atamna Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 2008 PMID: 17928358
Wischik Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8855335
Taniguchi Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 2005 PMID: 15611092
Hwang Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 1998 PMID: 9863642

Methylene Blue

Tocris Bioscience, part of Bio-Techne | Catalog # 3213

Key Product Details

Biological Activity for Methylene Blue

Technical Data for Methylene Blue

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Methylene Blue

Product Documents for Methylene Blue

COA

Certificate of Analysis

SDS

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