Methylene Blue

Name: Methylene Blue
Brand: Tocris Bioscience
SKU: 3213
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3213

(1)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Biological stain; also neuroprotective and antimalarial
Chemical Name:	3,7-bis(Dimethylamino)phenazathionium chloride
Molecular Weight:	319.85

View Full Technical Details for Methylene Blue

Biological Activity for Methylene Blue

Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC₅₀ values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC₅₀ = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.

Technical Data for Methylene Blue

M. Wt	319.85
Formula	C₁₆H₁₈ClN₃S
Storage	Store at RT
CAS Number	61-73-4
PubChem ID	6099
InChi Key	CXKWCBBOMKCUKX-UHFFFAOYSA-M
Smiles	CN(C)C1=CC=C2C(SC(C3=N2)=C/C(C=C3)=[N+](C)/C)=C1.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Methylene Blue

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	3.2	10
	DMSO	3.2	10

Preparing Stock Solutions for Methylene Blue

The following data is based on the product molecular weight 319.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.26 mL	156.32 mL	312.65 mL
0.5 mM	6.25 mL	31.26 mL	62.53 mL
1 mM	3.13 mL	15.63 mL	31.26 mL
5 mM	0.63 mL	3.13 mL	6.25 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Methylene Blue

References are publications that support the biological activity of the product.

Poteet Neuroprotective actions of methylene blue and its derivatives. PLoS One 2012 PMID: 23118969
Wen Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. J.Biol.Chem. 2011 PMID: 21454572
Atamna Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 2008 PMID: 17928358
Taniguchi Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 2005 PMID: 15611092
Hwang Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 1998 PMID: 9863642
Wischik Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8855335
Mayer Inhibition of nitric oxide synthesis by methylene blue. Biochem.Pharmacol. 1993 PMID: 7679577

Citations for Methylene Blue

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Methylene Blue (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Methylene blue was used to prepare Eosin Methylene Blue agar plate

Name: Anonymous

Species: Human

Methylene blue was used to prepare Eosin Methylene Blue (EMB) agar plate. Excellent product.

There are no reviews that match your criteria.