Methylene Blue

Product Description

Methylene Blue is a biological stain and redox indicator. Methylene blue inhibits monoamine oxidase A, tau filament formation and nitric oxide synthase (IC₅₀ values = 0.07 μM, 1.9 μM and 5.3 μM, respectively), and inhibits guanylate cyclase. Methylene Blue delays senescence and stimulates proliferation of fibroblasts and skin cells. In vitro, Methylene Blue promotes wound healing, upregulates elastin expression and production, increases synthesis of heme, is neuroprotective (EC₅₀ = 0.18 nM) and rescues the loss of dopaminergic neurons induced by Rotenone (Cat. No. 3616). Also antimalarial.

Solubility

Preparing Stock Solutions for Methylene Blue

The following data is based on the product molecular weight 319.85

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.26 mL	156.32 mL	312.65 mL
0.5 mM	6.25 mL	31.26 mL	62.53 mL
1 mM	3.13 mL	15.63 mL	31.26 mL
5 mM	0.63 mL	3.13 mL	6.25 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Poteet Neuroprotective actions of methylene blue and its derivatives. PLoS One 2012 PMID: 23118969
• Wen Alternative mitochondrial electron transfer as a novel strategy for neuroprotection. J.Biol.Chem. 2011 PMID: 21454572
• Mayer Inhibition of nitric oxide synthesis by methylene blue. Biochem.Pharmacol. 1993 PMID: 7679577
• Atamna Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 2008 PMID: 17928358
• Wischik Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines. Proc.Natl.Acad.Sci.U.S.A. 1996 PMID: 8855335
• Taniguchi Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins. J.Biol.Chem. 2005 PMID: 15611092
• Hwang Comparison of two soluble guanylyl cyclase inhibitors, methylene blue and ODQ, on sodium nitroprusside-induced relaxation in guinea-pig trachea. Br.J.Pharmacol. 1998 PMID: 9863642