Maleimide Activated R-Phycoerythrin

Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

Features	Benefits
Compatible protein thiolation kit available	No specialist knowledge required
Covalent bond	Highly stable conjugates
Maleimide activated labels can be used with a range of biomolecules, including oligonucleotides	Flexible
Scalable	Easy transfer from R&D to manufacturing
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Traditional, well-known conjugation chemistry	Trusted method

Maleimide activated labels

Maleimide - RPE

Maleimide - HRP

Maleimide - Alkaline Phosphatase

Maleimide - APC

Maleimide-Streptavidin

Maleimide-Ovalbumin

Maleimide-BSA

Maleimide - KLH

If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label:

1. Protein Thiolation Kit

Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein.

Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working.

2. Thiol Quantification Kit

The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction.

The assay is in a convenient 96-well plate format and contains all the necessary reagents required.

Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols.

This product is manufactured by Abcam and distributed by Novus Biologicals.