5X COOMASSIEnano Concentrate Protein Staining Solution
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33179
Key Product Details
Applications
Western Blot
Product Summary for 5X COOMASSIEnano Concentrate Protein Staining Solution
5X COOMASSIE nano, innovatively enhanced by proprietary nano-technology, is a concentrated, easy-to-reconstitute protein staining solution for SDS-PAGE gels. Coomassie blue protein staining is widely used in gel electrophoresis techniques for visualizing protein bands. This next generation Coomassie dye is formulated for quick protein detection and higher sensitivity. Additionally, 5X COOMASSIE nano eliminates the need for traditional destaining and wash steps. In the absence of hazardous substances such as methanol and acetic acid, 5X COOMASSIE nano is considered safe and environmentally friendly by reducing carbon footprint by as much as 80%. Besides western blot, this Coomassie protein stain is also compatible with mass spectrophotometry.
Key Features:
Quick Staining - Achieve vibrant blue staining results in minutesNo Destaining Required
No Washing Step Needed
Non-Hazardous Material - Ensures a safe and environmentally friendly staining experience
High Sensitivity
Product Specifications
Application Notes
There is no risk of excessive staining. Instead, superior visibility for each band is achieved when the gel is submerged overnight. Carefully mix COOMASSIE nano up and down few times before use. If the gel is thicker than mini-gel (1mm), it will take longer to incubate. The staining solution may contain blue clumps. This is normal and easily dissolved in distilled water
Scientific Data Images for 5X COOMASSIEnano Concentrate Protein Staining Solution
Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] -
Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] - COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.
Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] -
Western Blot: 5X COOMASSIEnano Concentrate Protein Staining Solution [NBP3-33179] - COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.Formulation, Preparation, and Storage
Preparation Method
Prepare a 5L bottle. Unpack and pour the liquid content into the bottle.Rinse residual reagent in the original bottle with deionized water. Add deionized water until the volume reaches 5L.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: 5X COOMASSIEnano Concentrate Protein Staining Solution
Alternate Names
COOMASSIE, Coomassie brilliant blue
Additional 5X COOMASSIEnano Concentrate Protein Staining Solution Products
Product Documents for 5X COOMASSIEnano Concentrate Protein Staining Solution
Product Specific Notices for 5X COOMASSIEnano Concentrate Protein Staining Solution
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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