Methoxy-X04
Tocris Bioscience, part of Bio-Techne | Catalog # 4920
Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging
Key Product Details
Description
Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging
Product Description
Key information: Methoxy-X04 is a blood-brain barrier penetrant fluorescent amyloid β (Aβ) probe. Suitable for in vivo imaging.Used for: the detection and quantification of Aβ plaques, tangles and cerebrovascular amyloid. Gives high resolution fluorescent imaging on individual plaques in live animals by 2-photon microscopy. Methoxy-X04 co-localizes with CD68+ phagosomes in plaque-associated Iba1+ microglia; and it labels molecularly distinct plaque-associated microglia populations.
Application: confocal microscopy, in vivo imaging.
Properties and Photophysical Data: Methoxy-X04 displays high in vitro binding affinity (Ki = 26.8 nM); binds selectively to fibrillar β-sheet deposits. Excitation and emission maxima (λ) are 370 nm and 452 nm, respectively.
Optical Data
| λabs | 370 nm |
| λem | 452 nm |
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Product Specifications
Molecular Weight
344.40
Formula
C23H20O3
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
4,4'-[(2-methoxy-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
CAS Number
863918-78-9
PubChem ID
16049314
InChI Key
FGYNZFHVGOFCMD-KHVHPYDTSA-N
SMILES
OC(C=C3)=CC=C3/C=C/C1=CC(OC)=C(/C=C/C2=CC=C(O)C=C2)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 34.44 | 100 | |
| Ethanol | 3.44 | 10 |
Preparing Stock Solutions
for Methoxy-X04
The following data is based on the product molecular weight 344.40
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.90 mL | 14.52 mL | 29.04 mL |
| 5 mM | 0.58 mL | 2.90 mL | 5.81 mL |
| 10 mM | 0.29 mL | 1.45 mL | 2.90 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.58 mL |
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Background References
References are publications that support the biological activity of the product.
- Heneka NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. Nature. 2013 PMID: 23254930
- Grubman Transcriptional signature in microglia associated with Aβ plaque phagocytosis. Nat.Commun. 2021 PMID: 34021136
- Yamanaka PPARγ/RXRα-induced and CD36-mediated microglial amyloid-β phagocytosis results in cognitive improvement in amyloid precursor protein/presenilin 1 mice. J.Neurosci. 2012 PMID: 23197723
- Klunk Imaging Aβ plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J.Neuropathol.Exp.Neurol. 2002 PMID: 12230326
- Bolmont Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. J.Neurosci. 2008 PMID: 18417708
Product Specific Notices for Methoxy-X04
For research use only
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