IRDye® 800CW, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 8880
Near-infrared dye (Reactive group: maleimide)
Key Product Details
Description
Near-infrared dye (Reactive group: maleimide)
Product Description
Key information: IRDye® 800CW, Maleimide is a near-infrared (NIR) fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 773 nm and 792 nm, respectively.
Please see the product protocol for further information and a guide to protein/antibody labeling.
It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL)
NOTE: When labeling a peptide or protein with IRDye® 800CW Maleimide, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e.
IRDye® is a registered trademark of Li-Cor and is used under license.
Optical Data
| λabs | 774 nm |
| λem | 789 nm |
| Extinction Coefficient (ε) | 240000 M-1cm-1 |
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Product Specifications
Molecular Weight
1191.25
Formula
C52H57N4Na3O16S4
Storage
Store at -20°C
Purity
≥80% (HPLC)
Chemical Name
Sodium 2-((E)-2-((E)-3-(2-((E)-1-(6-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate
CAS Number
1279564-25-8
InChI Key
CAXPRNLNWICSJV-UHFFFAOYSA-K
SMILES
CC1(C)C2=CC(S(=O)(O[Na])=O)=CC=C2[N+](CCCCS([O-])(=O)=O)=C1/C=C/C3=C(OC4=CC=C(S(=O)(O[Na])=O)C=C4)/C(CCC3)=C/C=C5C(C)(C)C(C=C(S(=O)(O[Na])=O)C=C6)=C6N\5CCCCCC(NCCN7C(C=CC7=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Product Specific Notices for IRDye® 800CW, Maleimide
For research use only
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