(Z)-Pugnac
Tocris Bioscience, part of Bio-Techne | Catalog # 3384
O-GlcNAcase and β-hexosaminidase inhibitor
Key Product Details
Description
O-GlcNAcase and β-hexosaminidase inhibitor
Product Description
(Z)-Pugnac is an O-GlcNAc-β-N-acetylglucosaminidase (O-GlcNAcase) and β-hexosaminidase inhibitor (Ki values are 46 and 36 nM respectively) that increases O-GlcNAc levels ~ 2-fold in HT29 cells. Z-linked isomer is more potent than the E isomer.Product Specifications
Molecular Weight
353.33
Formula
C15H19N3O7
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
O-(2-Acetamido-2-deoxy-D-glucopyranosylidene)amino-Z-N-phenylcarbamate
CAS Number
132489-69-1
PubChem ID
6074876
InChI Key
PBLNJFVQMUMOJY-JXAWBTAJSA-N
SMILES
OC(C1NC(C)=O)C(O)C(CO)O/C1=N\OC(NC2=CC=CC=C2)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.33 | 100 |
Preparing Stock Solutions
for (Z)-Pugnac
The following data is based on the product molecular weight 353.33
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.83 mL | 14.15 mL | 28.30 mL |
| 5 mM | 0.57 mL | 2.83 mL | 5.66 mL |
| 10 mM | 0.28 mL | 1.42 mL | 2.83 mL |
| 50 mM | 0.06 mL | 0.28 mL | 0.57 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Macauley O-GlcNAcase uses substrate-assisted catalysis. J.Biol.Chem. 2005 PMID: 15795231
- Perreira Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry. Bioorg.Med.Chem. 2006 PMID: 16214344
- Dong and Hart Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol. J.Biol.Chem. 1994 PMID: 8034696
Product Specific Notices for (Z)-Pugnac
For research use only
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