XIAP Tracer mF-Smac

Name: XIAP Tracer mF-Smac
Brand: Tocris Bioscience
SKU: 8069

Tocris Bioscience, part of Bio-Techne | Catalog # 8069

COA / SDS

Fluorescein labeled XIAP ligand for TR-FRET assay development

Key Product Details

Description:	Fluorescein labeled XIAP ligand for TR-FRET assay development
Purity:	≥95% (HPLC)
Molecular Weight:	989.1

View Full Technical Details for XIAP Tracer mF-Smac

Technical Data
Citations

Biological Activity for XIAP Tracer mF-Smac

XIAP Tracer mF-Smac is a fluorescent labeled peptide used as an acceptor dye with a terbium labeled BIR domain antibody in TR-FRET assays (K_d values are 4.7 nM at BIR3 (XIAP); 17.9 nM in XIAP polarization assay).

Optical Data for XIAP Tracer mF-Smac

Emission Color	Near-IR
λ_abs	485 nm
λ_em	520 nm
Closest Laser line	488 nm
Application	Fluorescence polarization (FP), Time-resolved fluorescence energy transfer (TR-FRET)

Technical Data for XIAP Tracer mF-Smac

M. Wt	989.1
Formula	C₅₁H₆₀N₁₀O₁₁
Single Letter Sequence	XRPFK (Modifications: X-1 = 2-Aminobutanoic acid, Lys-5 = Lys(5-FAM), Lys-5 = C-terminal amide)
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	783340-88-5
InChi Key	ONHYLEJPWIONKB-HECCNADXSA-N
Smiles	CC[C@H](N)C(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(N)=O)CCCCNC(C2=CC=C3C(C(OC34C5=CC=C(O)C=C5OC6=CC(O)=CC=C64)=O)=C2)=O)=O)CC7=CC=CC=C7)=O)=O)CCCNC(N)=N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for XIAP Tracer mF-Smac

Solubility

Soluble to 2mg/ml in 25% ethanol / water

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References for XIAP Tracer mF-Smac

References are publications that support the biological activity of the product.

Chaudhry Building homogeneous time-resolved fluorescence resonance energy transfer assays for characterization of bivalent inhibitors of an inhibitor of apoptosis protein target. Anal.Biochem. 2016 PMID: 26743718