WS 12

Name: WS 12
Brand: Tocris Bioscience
SKU: 3040
Rating: 5 (2 reviews)

Catalog # 3040 | Tocris Bioscience a Bio-Techne Brand

(2)

Citations (2)

COA / SDS

TRPM8 agonist; cooling agent

Key Product Details

Description:	TRPM8 agonist; cooling agent
Chemical Name:	(1R,2S)-N-(4-Methoxyphenyl)-5-methyl-2-(1-methylethyl)cyclohexanecarboxamide
Purity:	≥99% (HPLC)
Molecular Weight:	289.41

View Full Technical Details for WS 12

Technical Data
Citations (2) / Reviews (2)

Biological Activity for WS 12

WS 12 is a potent TRPM8 agonist that acts as a cooling agent (EC₅₀ = 193 nM).

Technical Data for WS 12

M. Wt	289.41
Formula	C₁₈H₂₇NO₂
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	68489-09-8
PubChem ID	11266244
InChi Key	HNSGVPAAXJJOPQ-XOKHGSTOSA-N
Smiles	C[C@H](C)[C@H]1[C@H]([C@@](NC2=CC=C(OC)C=C2)=O)C[C@H](C)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for WS 12

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	21.71	75

Preparing Stock Solutions for WS 12

The following data is based on the product molecular weight 289.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.75 mM	4.61 mL	23.04 mL	46.07 mL
3.75 mM	0.92 mL	4.61 mL	9.21 mL
7.5 mM	0.46 mL	2.30 mL	4.61 mL
37.5 mM	0.09 mL	0.46 mL	0.92 mL

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References for WS 12

References are publications that support the biological activity of the product.

Beck Prospects for prostate cancer imaging and therapy using high-affinity TRPM8 activators. Cell Calcium 2007 PMID: 16949669
Bodding Characterisation of TRPM8 as a pharmacophore receptor. Cell Calcium 2007 PMID: 17517434

Citations for WS 12 (2)

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Citations are publications that use Bio-Techne products. Selected citations for WS 12 include:

Morgan et al. (2015) Genetic variants affecting human TRPA1 or TRPM8 structure can be classified in vitro as 'well expressed', 'poorly expressed' or 'salvageable'. Biosci Rep. 35
PMID: 26330615
Liu et al. (2013) TRPM8 is the principal mediator of menthol-induced analgesia of acute and inflammatory pain. Pain. 154:2169
PMID: 23820004

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Customer Reviews for WS 12 (2)

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Activation of TRPM8 currents

Name: Anonymous

Species: Rat

Assay Type: In Vitro

WS12 was used to activate TRPM8 currents in dorsal root ganglia neurons through whole cell mode patch clamp.
WS12 as selective TRPM8 agonist and better than menthol.

Name: Hiteshkumar Soni

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: Mouse Mesenteric artery

TRP Ion channel, TRPM8 agonist in in vivo experiments in mouse and in vitro tissue, especially Mouse Mesenteric artery to check change in arterial diameter.

PMID: Not published yet

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