W-7 hydrochloride

Name: W-7 hydrochloride
Brand: Tocris Bioscience
SKU: 0369
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 0369

Citations (16)

(1)

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Key Product Details

Description:	Calmodulin antagonist. Inhibits myosin light chain kinase
Chemical Name:	N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	377.33

View Full Technical Details for W-7 hydrochloride

Biological Activity for W-7 hydrochloride

W-7 hydrochloride is a calmodulin antagonist. Inhibits Ca²⁺-calmodulin-dependent phosphodiesterase (IC₅₀ = 28 μM) and myosin light chain kinase (IC₅₀ = 51 μM).

Compound Libraries for W-7 hydrochloride

W-7 hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for W-7 hydrochloride

M. Wt	377.33
Formula	C₁₆H₂₁ClN₂O₂S.HCl
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	61714-27-0
PubChem ID	124887
InChi Key	OMMOSRLIFSCDBL-UHFFFAOYSA-N
Smiles	Cl.NCCCCCCNS(=O)(=O)C1=CC=CC2=C(Cl)C=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for W-7 hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	1.89	5 with gentle warming
	DMSO	37.73	100

Preparing Stock Solutions for W-7 hydrochloride

The following data is based on the product molecular weight 377.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.65 mL	13.25 mL	26.50 mL
5 mM	0.53 mL	2.65 mL	5.30 mL
10 mM	0.27 mL	1.33 mL	2.65 mL
50 mM	0.05 mL	0.27 mL	0.53 mL

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References for W-7 hydrochloride

References are publications that support the biological activity of the product.

Asano Divergent pharmacological effects of three calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W 7), chlorprom. and calmidazolium, on isometric tension development and myosin light chain phosphorylation in intact bovine trachea J.Pharmacol.Exp.Ther. 1989 PMID: 2810127
Itoh and Hidaka Direct interaction of calmodulin antagonists with Ca²⁺/calmodulin-dependent cyclic nucleotide phosphodiesterase. J.Biochem. 1984 PMID: 6099352
Hidaka N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc.Natl.Acad.Sci.U.S.A. 1981 PMID: 6945588
Tanaka and Hidaka Hydrophobic regions function in calmodulin-enzyme(s) interactions. J.Biol.Chem. 1980 PMID: 6254958

Citations for W-7 hydrochloride (16)

Showing 1 - 10 of 16 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for W-7 hydrochloride include:

Lopez et al. (2022) Ketamine exerts its sustained antidepressant effects via cell-type-specific regulation of Kcnq2. Neuron. 110:2283
PMID: 35649415
Schiff et al. (2019) P38 inhibition reverses TGFβ1 and TNFα-induced contraction in a model of proliferative vitreoretinopathy. Commun Biol. 2:162
PMID: 31069271
Haag et al. (2018) The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Rep. 24:2418
PMID: 30157434
Yanda et al. (2017) Histone deacetylase 6 inhibition reduces cysts by decreasing cAMP and Ca(2+) in knock-out mouse models of polycystic kidney disease. J Biol Chem. 292:17897
PMID: 28887310
Gray and Golowasch (2016) Voltage Dependence of a Neuromodulator-Activated Ionic Current. Eneuro. 3
PMID: 27257619
Yuan et al. (2015) Protein kinase G-regulated production of H2S governs oxygen sensing. Sci Signal. 8:ra37
PMID: 25900831
Hou et al. (2015) The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin. Apoptosis. 13:e1002233
PMID: 26334624
Bonnefond et al. (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. J Biol Chem. 20:535
PMID: 25627260
Araki et al. (2015) Rapid dispersion of SynGAP from synaptic spines triggers AMPA receptor insertion and spine enlargement during LTP. J Neurosci. 85:173
PMID: 25569349
Mukunda et al. (2014) Dimerisation of the Drosophila odorant coreceptor Orco. Neuron. 8:261
PMID: 25221476
Chang et al. (2013) Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition. Psychopharmacology (Berl). 34:1096
PMID: 23349021
Abdou et al. (2013) The calcium signaling pathway regulates leydig cell steroidogenesis through a transcriptional cascade involving the nuclear receptor NR4A1 and the steroidogenic acute regulatory protein. Endocrinology. 154:511
PMID: 23183170
Midkiff et al. (2011) Small molecule inhibitors of the Candida albicans budded-to-hyphal transition act through multiple signaling pathways. PLoS One. 6:e25395
PMID: 21966518
Dai et al. (2011) Phospholipase C, Ca2+, and calmodulin signaling are required for 5-HT2A receptor-mediated transamidation of Rac1 by transglutaminase. Front Cell Neurosci. 213:403
PMID: 20717650
Yanes and Romero (2009) Dihydrotestosterone stimulates aldosterone secretion by H295R human adrenocortical cells. Mol Cell Endocrinol. 303:50
PMID: 19428991
Long et al. (2009) Nerve Terminal GABAA Receptors Activate Ca2+/Calmodulin-dependent Signaling to Inhibit Voltage-gated Ca2+ Influx and Glutamate Release. Carcinogenesis. 284:8726
PMID: 19141616

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Customer Reviews for W-7 hydrochloride (1)

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PN and PH cells were treated with W-7 (catalog no. 0369) and W-13 (catalog no. 0361) purchased from Tocris.

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: PN and PH cells

PN and PH cells were treated with W-7 (catalog no. 0369) and W-13(catalog no. 0361) purchased from Tocris.

PMID: 28887310

There are no reviews that match your criteria.