VX 745

Name: VX 745
Brand: Tocris Bioscience
SKU: 3915

Tocris Bioscience, part of Bio-Techne | Catalog # 3915

Citations (21)

COA / SDS

Potent and selective p38α inhibitor

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Technical Data
Citations

Product Description
Product Specifications
Solubility
Preparing Stock Solutions
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Potent and selective p38α inhibitor

Alternative Names

VX745,VX-745,Neflamapimod

View all p38 Inhibitors »

Product Description

VX 745 is a highly potent and selective p38α/MAPK14 inhibitor, also inhibits p38β (IC₅₀ values are 10 nM and 220 nM respectively). VX 745 blocks TNFα production in LPS-stimulated HWB in vitro (IC₅₀ = 51-180 nM). Also reduces phosphorylation of p38MAPKα and the downstream target Hsp27, leading to vasodilation, reduces IL-1β levels and amyloid plaques in cortex and hippocampus and lowers CSF tau levels in vivo.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of VX 745 is reviewed on the chemical probes website.

Product Specifications

Molecular Weight

436.26

Formula

C₁₉H₉Cl₂F₂N₃OS

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-(2,6-Dichlorophenyl)-2-[2,4-difluorophenyl)thio]-6H-pyrimido[1,6-b]pyridazin-6-one

CAS Number

209410-46-8

PubChem ID

3038525

InChI Key

VEPKQEUBKLEPRA-UHFFFAOYSA-N

SMILES

O=C2N=CN1N=C(SC4=CC=C(F)C=C4F)C=CC1=C2C3=C(Cl)C=CC=C3Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	43.63	100

Preparing Stock Solutions for VX 745

The following data is based on the product molecular weight 436.26

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.29 mL	11.46 mL	22.92 mL
5 mM	0.46 mL	2.29 mL	4.58 mL
10 mM	0.23 mL	1.15 mL	2.29 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

Calculators

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Alam Selective brain-targeted antagonism of p38 MAPKα reduces hippocampal IL-1 levels and improves Morris Water Maze performance in aged rats. J.Alzheimers Dis. 2015 PMID: 26401942
Petrova p38 MAPK signalling regulates cytokine production in IL-33 stimulated type 2 innate lymphoid cells. Sci.Rep. 2020 PMID: 32103032
Pandey Neflamapimod induces vasodilation in resistance mesenteric arteries by inhibiting p38 MAPKα and downstream Hsp27 phosphorylation. Sci.Rep. 2022 PMID: 35318382
Duffy The discovery of VX-745: a novel and selective p38α kinase inhibitor. ACS Med.Chem.Lett. 2011 PMID: 24900264
Goldstein Selective p38alpha inhibitors clinically evaluated for the treatment of chronic inflammatory disorders. J.Med.Chem. 2010 PMID: 19950901
Bagley Rapid synthesis of VX-745: p38 MAP kinase inhibition in Werner syndrome cells. Bioorg.Med.Chem.Lett. 2007 PMID: 17659871