VVD-214 New

Name: VVD-214
Brand: Tocris Bioscience
SKU: 9027

Tocris Bioscience, part of Bio-Techne | Catalog # 9027

COA / SDS

Potent WRN covalent allosteric inhibitor; orally bioavailable

Key Product Details

Description:	Potent WRN covalent allosteric inhibitor; orally bioavailable
Alternative Names:	RO7589831
Chemical Name:	N-[(1S,2E)-1-Cyclopropyl-3-(methylsulfonyl)-2-propen-1-yl]-2-(1,1-difluoroethyl)-4-phenoxy-5-pyrimidinecarboxamide
Purity:	≥98% (HPLC)
Molecular Weight:	437.46

View Full Technical Details for VVD-214

Technical Data
Citations

Biological Activity for VVD-214

VVD-214 is a potent werner syndrome helicase (WRN) covalent allosteric inhibitor (IC₅₀ = 0.13 μM and 2.4 μM with and without ATP, respectively). The selective binding of VVD-214 to cysteine C727 of WRN triggers double-stranded DNA breaks, nuclear swellings, cell death and tumor regression in microsatellite instability-high (MSI-H) colorectal cancer models. Highly stable in hepatocytes and whole blood, VVD-214 is orally bioavailable.

Licensing Information

Sold with permission from Vividion Therapeutics

Technical Data for VVD-214

M. Wt	437.46
Formula	C₂₀H₂₁F₂N₃O₄S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	3026500-20-6
PubChem ID	38658751
InChi Key	UWPFUBNCDABUEE-SIFUEBAJSA-N
Smiles	CS(/C=C/[C@H](C1CC1)NC(C(C(OC2=CC=CC=C2)=N3)=CN=C3C(F)(F)C)=O)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for VVD-214

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	21.87	50

Preparing Stock Solutions for VVD-214

The following data is based on the product molecular weight 437.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.57 mL	22.86 mL	45.72 mL
2.5 mM	0.91 mL	4.57 mL	9.14 mL
5 mM	0.46 mL	2.29 mL	4.57 mL
25 mM	0.09 mL	0.46 mL	0.91 mL

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Reconstitution Calculator

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References for VVD-214

References are publications that support the biological activity of the product.

Baltgalvis Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase. Nature 2024 PMID: 38658751
Kikuchi Identification of VVD-214/RO7589831, a clinical-stage, covalent allosteric inhibitor of WRN helicase for the treatment of MSI-high cancers. J.Med.Chem. 2025 PMID: 40964743