UH15-38
Tocris Bioscience, part of Bio-Techne | Catalog # 8104
Potent and selective RIPK3 inhibitor; also blocks activation of necroptosis
Key Product Details
Description
Potent and selective RIPK3 inhibitor; also blocks activation of necroptosis
Product Description
UH15-38 is a potent and selective receptor interacting protein kinase 3 (RIPK3) inhibitor (IC50 = 20 nM). UH15-38 potently and selectively blocks influenza A-induced necroptosis in alveolar epithelial cells in vivo (IC50 = 39.5 nM), and prevents damage from lung inflammation without compromising antiviral adaptive immune responses or impeding viral clearance. Blocks TNFα-induced necroptosis in primary murine embryonic fibroblasts (IC50 = 98 nM) and mouse cell lines in vitro.Scientific Data Images for UH15-38
Published vs Bio-Techne batch comparison for UH15-38.
Inhibition of necroptosis by UH15-38 in human FADD-deficient Jurkat cells. Cells were treated with 10 ng/ml of human TNF alpha for 24 hr in the presence of the dose range of the inhibitors. Cell viability was determined using CellTiter-Glo assay (Promega). IC50 for the published molecule was 238.2 nM and 160.2 nM for the Bio-Techne batch. Data kindly provided by A. Degterev’s laboratory, University of Tufts.Licensing Information
Sold under license from Tufts UniversityProduct Specifications
Molecular Weight
514.45
Formula
C26H27N5O2.2HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
3-(3-Hydroxyphenyl)-1-methyl-7-[[3-(4-methyl-1-piperazinyl)phenyl]amino]-1,6-naphthyridin-2(1H)-one dihydrochloride
InChI Key
HIVPDYQXZCXJQM-UHFFFAOYSA-N
SMILES
O=C1C(C2=CC=CC(O)=C2)=CC3=C(N1C)C=C(NC4=CC=CC(N5CCN(CC5)C)=C4)N=C3.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 51.44 | 100 | |
| DMSO | 51.44 | 100 |
Preparing Stock Solutions
for UH15-38
The following data is based on the product molecular weight 514.45
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.72 mL | 19.44 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.89 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Gautam Necroptosis blockade prevents lung injury in severe influenza. Nature 2024 PMID: 38600381
Product Specific Notices for UH15-38
For research use only
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