TWS 119

Name: TWS 119
Brand: Tocris Bioscience
SKU: 7405

Tocris Bioscience, part of Bio-Techne | Catalog # 7405

Citations (9)

COA / SDS

Potent GSK3 inhibitor; induces neuronal and CD8(+) T cell differentiation

Key Product Details

Description:	Potent GSK3 inhibitor; induces neuronal and CD8(+) T cell differentiation
Chemical Name:	3-[[6-(3-Aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]phenol
Purity:	≥97% (HPLC)
Molecular Weight:	318.33

View Full Technical Details for TWS 119

Technical Data
Citations

Biological Activity for TWS 119

TWS 119 is a potent inhibitor of glycogen synthase kinase-3β (IC₅₀ = 30 nM). TWS 119 inhibits both GSK3α and GSK3β in vitro (IC₅₀ = 88 nM and 410 nM, respectively). TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM.

Compound Libraries for TWS 119

TWS 119 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for TWS 119

M. Wt	318.33
Formula	C₁₈H₁₄N₄O₂
Storage	Store at +4°C
Purity	≥97% (HPLC)
CAS Number	601514-19-6
PubChem ID	9549289
InChi Key	VPVLEBIVXZSOMQ-UHFFFAOYSA-N
Smiles	OC1=CC=CC(OC2=C3C(NC(C4=CC=CC(N)=C4)=C3)=NC=N2)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TWS 119

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	31.83	100
	ethanol	1.59	5

Preparing Stock Solutions for TWS 119

The following data is based on the product molecular weight 318.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.14 mL	15.71 mL	31.41 mL
5 mM	0.63 mL	3.14 mL	6.28 mL
10 mM	0.31 mL	1.57 mL	3.14 mL
50 mM	0.06 mL	0.31 mL	0.63 mL

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References for TWS 119

References are publications that support the biological activity of the product.

Gattinoni Wnt signaling arrests effector T cell differentiation and generates CD8+ memory stem cells. Nat.Med. 2009 PMID: 19525962
Forget Stimulation of Wnt/β-catenin pathway in human CD8+ T lymphocytes from blood and lung tumors leads to a shared young/memory phenotype. PLoS One 2012 PMID: 22859966
Zeng Glycogen synthase kinase 3 regulates PAX3-FKHR-mediated cell proliferation in human alveolar rhabdomyosarcoma cells. Biochem.Biophys.Res.Commun. 2009 PMID: 19995556