TP 064

Name: TP 064
Brand: Tocris Bioscience
SKU: 6008

Tocris Bioscience a Bio-Techne Brand

Catalog # 6008

Citations (3)

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Key Product Details

Description:	Potent and selective PRMT 4 inhibitor
Chemical Name:	N-Methyl-N-((2-(1-(2-(methylamino)ethyl)piperidin-4-yl)pyridin-4-yl)methyl)-3-phenoxybenzamide
Purity:	≥98% (HPLC)
Molecular Weight:	458.6

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Biological Activity for TP 064

TP 064 is a potent and selective PRMT4 inhibitor; inhibits methylation of H3 (1-25) and MED12 (IC₅₀ values are 100-fold selectivity over other histone methyltransferases and non-epigenetic targets. Inhibits proliferation of certain multiple myeloma cell lines, arresting cells in G1 phase. TP 064 can be used in vivo. TP 064 inhibits the monocyte/macrophage-driven inflammation in ex vivo.

To request the negative control for TP 064, please fill out the TP 064N request form on the SGC website..

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the TP 064 probe summary on the SGC website.

Compound Libraries for TP 064

TP 064 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for TP 064

M. Wt	458.6
Formula	C₂₈H₃₄N₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2080306-20-1
PubChem ID	122707126
InChi Key	VUIITYLFSAXKIQ-UHFFFAOYSA-N
Smiles	O=C(N(C)CC1=CC(C2CCN(CCNC)CC2)=NC=C1)C3=CC=CC(OC4=CC=CC=C4)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TP 064

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	45.86	100
	DMSO	45.86	100

Preparing Stock Solutions for TP 064

The following data is based on the product molecular weight 458.6.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.18 mL	10.90 mL	21.81 mL
5 mM	0.44 mL	2.18 mL	4.36 mL
10 mM	0.22 mL	1.09 mL	2.18 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for TP 064

References are publications that support the biological activity of the product.

Zhang PRMT4 inhibitor TP-064 inhibits the pro-inflammatory macrophage lipopolysaccharide response in vitro and ex vivo and induces peritonitis-associated neutrophilia in vivo. Biochim.Biophys.Acta Mol.Basis Dis. 2021 PMID: 34311083
Scheer A chemical biology toolbox to study protein methyltransferases and epigenetic signaling. Nat.Commun. 2019 PMID: 30604761
Nakayama TP-064, a potent and selective small molecule inhibitor of PRMT4 for multiple myeloma. Oncotarget 2018 PMID: 29719619

Citations for TP 064 (3)

Showing 1 - 3 of 3 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for TP 064 include:

Clemons et al. (2022) Protein arginine methyltransferase 4 modulates nitric oxide synthase uncoupling and cerebral blood flow in Alzheimer's disease J Cell Physiol. 2022;
PMID: 36036549
Zhang et al. (2021) PRMT4 inhibitor TP-064 inhibits the pro-inflammatory macrophage lipopolysaccharide response in vitro and ex vivo and induces peritonitis-associated neutrophilia in vivo. Biochim.Biophys.Acta Mol.Basis Dis.. 1867:166212
PMID: 34311083
Zhong et al. (2018) CARM1 Methylates GAPDH to Regulate Glucose Metabolism and Is Suppressed in Liver Cancer. Cell Rep. 24:3207
PMID: 30232003

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