TBB

Name: TBB
Brand: Tocris Bioscience
SKU: 2275
Price: 129 EUR

Tocris Bioscience a Bio-Techne Brand

Catalog # 2275

Citations (6)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Selective cell-permeable CK2 inhibitor
Alternative Names:	NSC 231634
Chemical Name:	4,5,6,7-Tetrabromobenzotriazole
Purity:	≥98% (HPLC)
Molecular Weight:	434.71

View Full Technical Details for TBB

Biological Activity for TBB

TBB is a cell-permeable, selective inhibitor of casein kinase-2 (CK2) (IC₅₀ = 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively). Exhibits modest discrimination between CK2 subunits, with K_i values ranging from 80 nM to 210 nM. Acts in an ATP/GTP-competitive manner and displays one to two orders of magnitude selectivity over a panel of 33 protein kinases.

Compound Libraries for TBB

TBB is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for TBB

M. Wt	434.71
Formula	C₆HBr₄N₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	17374-26-4
PubChem ID	1694
InChi Key	OMZYUVOATZSGJY-UHFFFAOYSA-N
Smiles	BrC1=C2NN=NC2=C(Br)C(Br)=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TBB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	43.47	100

Preparing Stock Solutions for TBB

The following data is based on the product molecular weight 434.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.30 mL	11.50 mL	23.00 mL
5 mM	0.46 mL	2.30 mL	4.60 mL
10 mM	0.23 mL	1.15 mL	2.30 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for TBB

References are publications that support the biological activity of the product.

Zien Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2. Biochim.Biophys.Acta. 2005 PMID: 16203192
Pagano Optimization of protein kinase CK2 inhibitors derived from 4,5,6,7-tetrabromobenzimidazole. J.Med.Chem. 2004 PMID: 15566294
Sarno Selectivity of 4,5,6,7-tetrabromobenzotriazole, an ATP site-directed inhibitor of protein kinase CK2 ('casein kinase-2'). FEBS Lett. 2001 PMID: 11343704

Citations for TBB (6)

Showing 1 - 6 of 6 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for TBB include:

Lezmy (2017) M-current inhibition rapidly induces a unique CK2-dependent plasticity of the axon initial segment. Proc Natl Acad Sci U S A. 114(47):E10234
PMID: 29109270
Bartley et al. (2016) Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP. Eneuro. 3
PMID: 27957526
Korb et al. (2015) BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice. Sci Rep. 18:1464
PMID: 26301327
Navasa et al. (2015) Ikaros mediates the DNA methylation-independent silencing of MCJ/DNAJC15 gene expression in macrophages. J Urol. 5:14692
PMID: 26419808
Wu et al. (2014) The BCL2L1 and PGAM5 axis defines hypoxia-induced receptor-mediated mitophagy. Autophagy. 10:1712
PMID: 25126723
Buchanan et al. (2010) Facilitation of long-term potentiation by muscarinic M(1) receptors is mediated by inhibition of SK channels. Neuron. 68:948
PMID: 21145007

There are no citations that match your criteria.