SPRi 3

Product Description

SPRi 3 is a potent sepiapterin reductase (SPR) inhibitor (IC₅₀ = 53 - 74 nM for human SPR). Inhibits BH4 synthesis pathway and attenuates proliferation in naive CD4⁺ T cells. Suppresses proliferation of human effector CD4⁺ T cells following stimulation. Reduces SPR activity in mouse primary cultures of sensory neurons (IC₅₀ = 450 nM). Ameliorates colitis, suppressing the intestinal infiltration of T cells and other immune cells in vivo. Also reduces immune-cell infiltration into mouse lungs. Reduces neuropathic and inflammatory pain in mouse models.

Licensing Information

Sold under license from the Children's Medical Center Corporation.

Solubility

Preparing Stock Solutions for SPRi 3

The following data is based on the product molecular weight 262.30

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.81 mL	19.06 mL	38.12 mL
5 mM	0.76 mL	3.81 mL	7.62 mL
10 mM	0.38 mL	1.91 mL	3.81 mL
50 mM	0.08 mL	0.38 mL	0.76 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Latremoliere Reduction of neuropathic and inflammatory pain through inhibition of the tetrahydrobiopterin pathway. Neuron 2015 PMID: 26087165
• Cronin The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature 2018 PMID: 30405245
• Haruki Tetrahydrobiopterin biosynthesis as a potential target of the kynurenine pathway metabolite xanthurenic acid. J.Biol.Chem. 2016 PMID: 26565027