Spautin 1

Name: Spautin 1
Brand: Tocris Bioscience
SKU: 5197

Tocris Bioscience, part of Bio-Techne | Catalog # 5197

Citations (11)

COA / SDS

USP10 and USP13 inhibitor; inhibits autophagy

Key Product Details

Description:	USP10 and USP13 inhibitor; inhibits autophagy
Chemical Name:	6-Fluoro-N-[(4-Fluorophenyl)methyl]-4-quinazolinamine
Purity:	≥98% (HPLC)
Molecular Weight:	271.26

View Full Technical Details for Spautin 1

Technical Data
Citations

Biological Activity for Spautin 1

Spautin 1 is an inhibitor of autophagy; inhibits USP10 and USP13 activity (IC₅₀ values are 0.6 and 0.7 μM respectively) and promotes degradation of Vps34 (class III PI 3-kinase) complexes. Selectively promotes apoptosis of cancer cells under starvation conditions. Prevents the PDGF-induced conversion from the contractile to synthetic phenotype in vascular smooth muscle cells.

Compound Libraries for Spautin 1

Spautin 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Spautin 1

M. Wt	271.26
Formula	C₁₅H₁₁F₂N₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1262888-28-7
PubChem ID	51037431
InChi Key	AWIVHRPYFSSVOG-UHFFFAOYSA-N
Smiles	FC1=CC=C(N=CN=C2NCC3=CC=C(F)C=C3)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Spautin 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	27.13	100

Preparing Stock Solutions for Spautin 1

The following data is based on the product molecular weight 271.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.69 mL	18.43 mL	36.87 mL
5 mM	0.74 mL	3.69 mL	7.37 mL
10 mM	0.37 mL	1.84 mL	3.69 mL
50 mM	0.07 mL	0.37 mL	0.74 mL

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References for Spautin 1

References are publications that support the biological activity of the product.

Liu Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 2011 PMID: 21962518
Salabei PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem.J. 2013 PMID: 23421427