SMIFH2

Name: SMIFH2
Brand: Tocris Bioscience
SKU: 4401

Tocris Bioscience a Bio-Techne Brand

Catalog # 4401

Citations (9)

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Key Product Details

Description:	FH2 domain inhibitor; prevents actin nucleation
Chemical Name:	1-(3-Bromophenyl)-5-(2-furanylmethylene)dihydro-2-thioxo-4,6(1H,5H)-pyrimidinedione
Purity:	≥99% (HPLC)
Molecular Weight:	377.21

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Biological Activity for SMIFH2

SMIFH2 is an inhibitor of formin homology 2 (FH2) domains. Prevents formin-mediated actin nucleation and barbed end elongation. Disrupts formin-dependent actin cytoskeletal structures in fission yeast and mammalian NIH 3T3 fibroblasts.

Technical Data for SMIFH2

M. Wt	377.21
Formula	C₁₅H₉BrN₂O₃S
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	340316-62-3
PubChem ID	2258538
InChi Key	MVFJHEQDISFYIS-XYOKQWHBSA-N
Smiles	O=C(/C(C(N2)=O)=C/C1=CC=CO1)N(C3=CC=CC(Br)=C3)C2=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SMIFH2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	37.72	100

Preparing Stock Solutions for SMIFH2

The following data is based on the product molecular weight 377.21.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.65 mL	13.26 mL	26.51 mL
5 mM	0.53 mL	2.65 mL	5.30 mL
10 mM	0.27 mL	1.33 mL	2.65 mL
50 mM	0.05 mL	0.27 mL	0.53 mL

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References for SMIFH2

References are publications that support the biological activity of the product.

Poincloux Contractility of the cell rear drives invasion of breast tumor cells in 3D Matrigel. Proc.Natl.Acad.Sci.USA 2011 PMID: 21245302
Rizvi Identification and characterization of a small molecule inhibitor of formin-mediated actin assembly. Chem.Biol. 2009 PMID: 19942139

Citations for SMIFH2 (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for SMIFH2 include:

Alieva et al. (2019) Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. Nat Commun. 10:3593
PMID: 31399564
Logue et al. (2018) c-Src activity is differentially required by cancer cell motility modes. Oncogene. 37:2104
PMID: 29379163
Ford et al. (2018) Chlamydia exploits filopodial capture and a macropinocytosis-like pathway for host cell entry. PLoS Pathog. 14:e1007051
PMID: 29727463
Ray et al. (2018) Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping. Proc Natl Acad Sci U S A. 115:E11568
PMID: 30459275
LeCorgne et al. (2018) Differential Toxicity of mDia Formin-Directed Functional Agonists and Antagonists in Developing Zebrafish. Front Pharmacol. 9:340
PMID: 29692731
Sepúlveda-Ramírez et al. (2018) Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo. Dev Biol. 437:140
PMID: 29555242
Basu et al. (2016) Arp2/3 and VASP Are Essential for Fear Memory Formation in Lateral Amygdala. Eneuro. 3
PMID: 27957528
Tornavaca et al. (2015) ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. J Neurosci. 208:821
PMID: 25753039
Ganguly et al. (2015) A dynamic formin-dependent deep F-actin network in axons. J Cell Biol. 210:401
PMID: 26216902

There are no citations that match your criteria.