SANT-1
Tocris Bioscience, part of Bio-Techne | Catalog # 1974
Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Key Product Details
Description
Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity
Product Description
SANT-1 is a potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (KD = 1.2 nM). Inhibits smoothened agonist effects with an IC50 of 20 nM (in Shh-LIGHT2 cells). Used in protocols to generate pancreatic β cells from hPSCs (see our protocol below).For more information about how SANT-1 may be used, see our protocol: Generation of β cells from hPSCs
Product Specifications
Molecular Weight
373.50
Formula
C23H27N5
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine
CAS Number
304909-07-7
PubChem ID
6878030
InChI Key
FOORCIAZMIWALX-JJIBRWJFSA-N
SMILES
CC1=NN(C(C)=C1\C=N\N1CCN(CC2=CC=CC=C2)CC1)C1=CC=CC=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.68 | 50 |
Preparing Stock Solutions
for SANT-1
The following data is based on the product molecular weight 373.50
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 5.35 mL | 26.77 mL | 53.55 mL |
| 2.5 mM | 1.07 mL | 5.35 mL | 10.71 mL |
| 5 mM | 0.54 mL | 2.68 mL | 5.35 mL |
| 25 mM | 0.11 mL | 0.54 mL | 1.07 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Chen Small molecule modulation of smoothened activity. Proc.Natl.Acad.Sci.USA.
Product Specific Notices for SANT-1
For research use only
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