SALAD1 New

Product Description

Key information: SALAD1 is a specific and high-affinity aptamer-ligand for aptamer Mango II (K_Dapp = 0.69 nM). Green fluorogenic dye displaying 3.5-fold brighter fluorescence than other Mango aptamer dyes such as TO1-biotin. Binds 7.5-fold more tightly to Mango II compared to TO dye. Exhibits a binding preference to its target RNA (Mango II) over the other three Mango aptamers (7-, 20- and 16-fold tighter than Mango I, III and IV). The improved brightness and tighter binding of SALAD1 to Mango aptamers allow detection and tracking of RNA with greater sensitivity and spatial resolution, facilitating studies of RNA localization, dynamics, and expression in live-cell and single-molecule imaging applications.

Used for: imaging RNA in live and fixed cells, even at low concentration.

Application: confocal cellular imaging.

Properties and Photophysical Data: excitation and emission maxima (λ) measured in Mango selection buffer are 511 nm and 540 nm, respectively; quantum yield QY = 0.722; extinction coefficient ε_(complex) = 47,284 M^-1cm^-1; fluorescence enhancement F_E = 1,500; fluorescence efficiency E = 2,170 nM^-1; brightness = 34,139 M^-1cm^-1. SALAD1 is cell-permeable.

Optical Data

Solubility

Preparing Stock Solutions for SALAD1

The following data is based on the product molecular weight 635.64

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	15.73 mL	78.66 mL	157.32 mL
0.5 mM	3.15 mL	15.73 mL	31.46 mL
1 mM	1.57 mL	7.87 mL	15.73 mL
5 mM	0.31 mL	1.57 mL	3.15 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Yang Structure-informed design of an ultrabright RNA-activated fluorophore. Nat.Chem. 2025 PMID: 40437193