SAHA

Name: SAHA
Brand: Tocris Bioscience
SKU: 4652
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 4652

Citations (3)

(1)

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Key Product Details

Description:	Class I and II HDAC inhibitor
Alternative Names:	Vorinostat
Chemical Name:	N-Hydroxy-N'-phenyloctanediamide
Purity:	≥98% (HPLC)
Molecular Weight:	264.32

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Biological Activity for SAHA

SAHA inhibits Class I and II histone deacetylases (HDACs); induces accumulation of acetylated histones H2A, H2B, H3 and H4 in transformed cultured cells. Suppresses cell growth in a range of cancer cell lines; induces apoptosis in cutaneous T cell lymphoma cells in vitro. Activates autophagy. SAHA increases efficiency of transcription factor-induced reprogramming of mouse embryonic fibroblasts (MEF) to induced pluripotent stem cells (iPSC). Also enhances adeno-associated virus transduction of HeLa cells.

Compound Libraries for SAHA

SAHA is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library, Tocriscreen Epigenetics Library, Tocriscreen FDA-Approved Drugs and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for SAHA

M. Wt	264.32
Formula	C₁₄H₂₀N₂O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	149647-78-9
PubChem ID	5311
InChi Key	WAEXFXRVDQXREF-UHFFFAOYSA-N
Smiles	O=C(CCCCCCC(NO)=O)NC1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SAHA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	26.43	100

Preparing Stock Solutions for SAHA

The following data is based on the product molecular weight 264.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.78 mL	18.92 mL	37.83 mL
5 mM	0.76 mL	3.78 mL	7.57 mL
10 mM	0.38 mL	1.89 mL	3.78 mL
50 mM	0.08 mL	0.38 mL	0.76 mL

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References for SAHA

References are publications that support the biological activity of the product.

Galluzzi Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 2017 PMID: 28529316
Nicolson Identification and validation of small molecules that enhance recombinant adeno-associated virus transduction following high-throughput screens. J.Virol. 2016 PMID: 27147738
Huangfu Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat.Biotechnol. 2008 PMID: 18568017
Marks and Breslow Dimethyl sulfoxide to vorinostat: development of this histone deacetylase inhibitor as an anticancer drug. Nat.Biotechnol. 2007 PMID: 17211407
Leoni The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines. Proc.Natl.Acad.Sci.U.S.A 2002 PMID: 11867742
Butler Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses the growth of prostate cancer cells in vitro and in vivo. Cancer Res. 2000 PMID: 11016644

Citations for SAHA (3)

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Citations are publications that use Bio-Techne products. Selected citations for SAHA include:

Ng-Blichfeldt et al. (2018) Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation. EBioMedicine. 36:461
PMID: 30236449
Bhattacharya et al. (2017) RAD51 interconnects between DNA replication, DNA repair and immunity. Nucleic Acids Res. 45:4590
PMID: 28334891
Sarenac et al. (2016) Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in humankeratocytes in vitro Scientific Reports. 6:34373
PMID: 27687492

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Excellent histone deacetylase inhibitor

Name: Luiz Alexandre Magno

Species: Rat

Assay Type: In Vitro

Cell Line/Tissue: PC12

Our lab uses this drug to monitor transcriptional activity of genes that depend on class I, II and IV of histone deacetylase. In the experiment shown in the figure, we have treated PC12 cells with 3 different concentrations of SAHA for 3h. We found increased transcriptional activity of PI3K gene upon SAHA treatment in a dose-dependent manner.

SAHA can be easily resuspended in DMSO. Make a stock solution with a concentration that will not exceed 0.01% of DMSO in the cell culture to avoid toxicity.

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