Ro 3306

Name: Ro 3306
Brand: Tocris Bioscience
SKU: 4181

Tocris Bioscience, part of Bio-Techne | Catalog # 4181

Citations (105)

COA / SDS

Cdk1 inhibitor

Key Product Details

Description:	Cdk1 inhibitor
Chemical Name:	5-(6-Quinolinylmethylene)-2-[(2-thienylmethyl)amino]-4(5H)-thiazolone
Purity:	≥98% (HPLC)
Molecular Weight:	351.45

View Full Technical Details for Ro 3306

Technical Data
Citations

Biological Activity for Ro 3306

Ro 3306 is an ATP-competitive cyclin-dependent kinase 1 (cdk1) inhibitor (K_i values range from 35 to 240 nM depending on cdk1 binding partner). Also reported to inhibit other cdks (K_i values are 0.89-1.32, 0.03 and >2 μM for cdk2, cdk3 and cdk4, respectively). Induces G2/M phase cell cycle arrest and apoptosis. Downregulates the expression of antiapoptotic proteins such as Bcl-2 and survivin and enhances downstream p53 signaling in acute myeloid leukemia (AML). RO 3306 also improves homology-directed repair (HDR) -mediated gene editing in hematopoietic stem and progenitor cells.

Compound Libraries for Ro 3306

Ro 3306 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Ro 3306

M. Wt	351.45
Formula	C₁₈H₁₃N₃OS₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	872573-93-8
PubChem ID	11631681
InChi Key	XOLMRFUGOINFDQ-YBEGLDIGSA-N
Smiles	O=C2/C(SC(NCC3=CC=CS3)=N2)=C/C1=CC=C(N=CC=C4)C4=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ro 3306

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.03	20 with gentle warming

Preparing Stock Solutions for Ro 3306

The following data is based on the product molecular weight 351.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.23 mL	71.13 mL	142.27 mL
1 mM	2.85 mL	14.23 mL	28.45 mL
2 mM	1.42 mL	7.11 mL	14.23 mL
10 mM	0.28 mL	1.42 mL	2.85 mL

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References for Ro 3306

References are publications that support the biological activity of the product.

Azhagiri Homology-directed gene-editing approaches for hematopoietic stem and progenitor cell gene therapy. Stem Cell Res.Ther. 2021 PMID: 34503562
Jorda How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 2018 PMID: 30234987
Vassilev Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc.Natl.Acad.Sci. 2006 PMID: 16818887
Yu CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage. Cancer Res. 2012 PMID: 22962268
Kojima The CDK1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML. Cancer Sci. 2009 PMID: 19385969