Ro 25-6981 maleate

Name: Ro 25-6981 maleate
Brand: Tocris Bioscience
SKU: 1594
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 1594

Citations (29)

(1)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	GluN2B-selective NMDA antagonist
Chemical Name:	(αR,βS)-α-(4-Hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidinepropanol maleate
Purity:	≥98% (HPLC)
Molecular Weight:	455.55

View Full Technical Details for Ro 25-6981 maleate

Complete Your Research

Biological Activity for Ro 25-6981 maleate

Ro 25-6981 maleate is a potent and selective activity-dependent blocker of NMDA receptors containing the GluN2B (formally NR2B) subunit. IC₅₀values are 0.009 and 52 μM for cloned receptor subunit combinations GluN1C/GluN2B and GluN1C/GluN2A respectively. Displays neuroprotectant effects in vivo and in vitro.

Please refer to IUPHAR Guide to Pharmacology for the most recent naming conventions.

Compound Libraries for Ro 25-6981 maleate

Ro 25-6981 maleate is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Ro 25-6981 maleate

M. Wt	455.55
Formula	C₂₂H₂₉NO₂.C₄H₄O₄
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	1312991-76-6
PubChem ID	53250677
InChi Key	FYJZEHCQSUBZDY-SEELMCCHSA-N
Smiles	OC(=O)\C=C/C(O)=O.C[C@@H](CN1CCC(CC2=CC=CC=C2)CC1)[C@@H](O)C1=CC=C(O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Ro 25-6981 maleate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	4.56	10 with gentle warming
	DMSO	45.55	100

Preparing Stock Solutions for Ro 25-6981 maleate

The following data is based on the product molecular weight 455.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.20 mL	10.98 mL	21.95 mL
5 mM	0.44 mL	2.20 mL	4.39 mL
10 mM	0.22 mL	1.10 mL	2.20 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Ro 25-6981 maleate

References are publications that support the biological activity of the product.

Kosowski and Liljequist The NR2B- selective N-MthD.-aspartate receptor antagonist Ro 25-6981 [(±)-(R*,S*)-α-(4-hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidine propanol] potentiates the effect of nicotine on locomotor activity and DA release J.Pharmacol.Exp.Ther. 2004 PMID: 15256539
Lynch Pharmacological characterization of interactions of RO 25-6981 with the NR2B (ε2) subunit. Eur.J.Pharmacol. 2001 PMID: 11290368
Fischer Ro 25-6981, a highly potent and selective blocker of N-MthD.-aspartate receptors containing the NR2B subunit. Characterization in vitro. J.Pharmacol.Exp.Ther. 1997 PMID: 9400004

Citations for Ro 25-6981 maleate (29)

Showing 1 - 10 of 29 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Ro 25-6981 maleate include:

Lassus et al. (2018) Glutamatergic and dopaminergic modulation of cortico-striatal circuits probed by dynamic calcium imaging of networks reconstructed in microfluidic chips. Sci Rep. 8:17461
PMID: 30498197
France (2017) Multiple roles of GluN2B-containing NMDA receptors in synaptic plasticity in juvenile hippocampus. Neuropharmacology. 112:76
PMID: 27523302
Bosse et al. (2017) Adenylyl Cyclase 1 Is Required for Ethanol-Induced Locomotor Sensitization and Associated Increases in NMDA Receptor Phosphorylation and Function in the Dorsal Medial Striatum. J Pharmacol Exp Ther. 363:148
PMID: 28838956
Volkmann et al. (2016) MPX-004 and MPX-007: New Pharmacological Tools to Study the Physiology of NMDA Receptors Containing the GluN2A Subunit. PLoS One. 11:e0148129
PMID: 26829109
Chen et al. (2016) β-arrestin-2 regulates NMDA receptor function in spinal lamina II neurons and duration of persistent pain. Nature Communications. 7:12531
PMID: 27538456
McQuail et al. (2016) NR2A-Containing NMDARs in the Prefrontal Cortex Are Required for Working Memory and Associated with Age-Related Cognitive Decline. J Neurosci. 36:12537
PMID: 27807032
Lauderdale et al. (2015) Osmotic Edema Rapidly Increases Neuronal Excitability Through Activation of NMDA Receptor-Dependent Slow Inward Currents in Juvenile and Adult Hippocampus. ASN Neuro. 7
PMID: 26489684
Tindi et al. (2015) ANKS1B Gene Product AIDA-1 Controls Hippocampal Synaptic Transmission by Regulating GluN2B Subunit Localization. J Neurosci. 35:8986
PMID: 26085624
Smith et al. (2014) A role for picomolar concentrations of pregnenolone sulfate in synaptic activity-dependent Ca²⁺ signaling and CREB activation. Mol Pharmacol. 86:390
PMID: 25057049
Jiménez-Sánchez et al. (2014) The role of GluN2A and GluN2B subunits on the effects of NMDA receptor antagonists in modeling schizophrenia and treating refractory depression. Neuropsychopharmacology. 39:2673
PMID: 24871546
Trepanier et al. (2013) Group II metabotropic glutamate receptors modify N-MthD.-aspartate receptors via Src kinase. Sci Rep. 3:926
PMID: 23378895
Hamida et al. (2013) Protein tyrosine phosphatase α in the dorsomedial striatum promotes excessive ethanol-drinking behaviors. Transl Psychiatry. 33:14369
PMID: 24005290
Louderback et al. (2013) Knockdown of BNST GluN2B-containing NMDA receptors mimics the actions of KA on novelty-induced hypophagia. Psychoneuroendocrinology. 3:e331
PMID: 24301649
Urban et al. (2013) Treatment with a clinically-relevant dose of methylphen. alters NMDA receptor composition and synaptic plasticity in the juvenile rat prefrontal cortex. Mol Brain. 101:65
PMID: 23333502
Koeglsperger et al. (2013) Impaired glutamate recycling and GluN2B-mediated neuronal calcium overload in mice lacking TGF-β1 in the CNS. Glia. 61:985
PMID: 23536313
Liu et al. (2013) Long-term potentiation of synaptic transmission in the adult mouse insular cortex: multielectrode array recordings. J Neurophysiol. 110:505
PMID: 23636718
Hoshiko et al. (2012) Deficiency of the microglial receptor CX3CR1 impairs postnatal functional development of thalamocortical synapses in the barrel cortex. J Neurosci. 32:15106
PMID: 23100431
Olsen and Sheng (2012) NMDA receptors and BAX are essential for Aβ impairment of LTP. Sci Rep. 2:225
PMID: 22355739
Bortolato et al. (2012) NMDARs mediate the role of monoamine oxidase A in pathological aggression. J Neurosci. 32:8574
PMID: 22723698
Nebieridze et al. (2012) β-OE unmasks metabotropic receptor-mediated metaplasticity of NMDA receptor transmission in the female rat dentate gyrus. PLoS One. 37:1845
PMID: 22541715
Ishima and Hashimoto (2012) Potentiation of nerve growth factor-induced neurite outgrowth in PC12 cells by ifenprodil: the role of σ-1 and IP3 receptors. Neurobiol Learn Mem. 7:e37989
PMID: 22655093
Noel et al. (2011) Tissue plasminogen activator is required for the development of fetal alcohol syndrome in mice. Proc Natl Acad Sci U S A. 108:5069
PMID: 21383198
Xie et al. (2011) Dependence of NMDA/GSK-3β mediated metaplasticity on TRPM2 channels at hippocampal CA3-CA1 synapses. J Neurosci. 4:44
PMID: 22188973
Terasaki et al. (2010) Activation of NR2A receptors induces ischemic tolerance through CREB signaling. J Cereb Blood Flow Metab. 30:1441
PMID: 20145658
Beazely et al. (2009) Platelet-derived growth factor selectively inhibits NR2B-containing N-MthD.-aspartate receptors in CA1 hippocampal neurons. J Biol Chem. 284:8054
PMID: 19106110
Hellier et al. (2009) NMDA receptor-mediated long-term alterations in epileptiform activity in experimental chronic epilepsy. Neuropharmacology. 56:414
PMID: 18930747
Popp et al. (2008) Cerebellar granule cells cultured from adolescent rats express functional NMDA receptors: an in vitro model for studying the developing cerebellum. J Neurochem. 106:900
PMID: 18466339
Niswender et al. (2008) Discovery, characterization, and antiparkinsonian effect of novel positive allosteric modulators of metabotropic glutamate receptor 4. Mol Pharmacol. 74:1345
PMID: 18664603
Ge et al. (2007) A critical period for enhanced synaptic plasticity in newly generated neurons of the adult brain. Neuron. 54:559
PMID: 17521569

There are no citations that match your criteria.

Showing 1 - 10 of 29 citations Showing All

Customer Reviews for Ro 25-6981 maleate (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Effect of Ro 25-6981 maleate on NMDA-receptor mediated current in the basolateral amygdala following chronic ethanol exposure

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Cell Line/Tissue: Basolateral Amygdala Slices

We used this drug to block NMDA receptors containing the GluN2B subunit on pyramidal neurons in the basolateral amygdala (BLA). We recorded from coronal sections of BLA and bath applied Ro 25-6981 maleate for 10 minutes.

PMID: 29953905

There are no reviews that match your criteria.