Rhosin hydrochloride

Name: Rhosin hydrochloride
Brand: Tocris Bioscience
SKU: 5003

Tocris Bioscience, part of Bio-Techne | Catalog # 5003

Citations (39)

COA / SDS

Rho inhibitor

Key Product Details

Description:	Rho inhibitor
Chemical Name:	D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	394.86

View Full Technical Details for Rhosin hydrochloride

Technical Data
Citations

Biological Activity for Rhosin hydrochloride

Rhosin hydrochloride is a Rho GTPase inhibitor (K_d = 354 nM). Inhibits binding of RhoA to guanine nucleotide exchange factors (GEFs) including LARG, DBL, LBC, p115 RhoGEF and PDZ RhoGEF. Has no effect on the binding of Rac1 or Cdc42 to their respective GEFs. Suppresses RhoA/C-mediated breast cancer cell migration and invasion in vitro. Also induces neurite outgrowth in synergy with NGF.

Technical Data for Rhosin hydrochloride

M. Wt	394.86
Formula	C₂₀H₁₈N₆O.HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1281870-42-5
PubChem ID	44662615
InChi Key	SFRGBDFQSLZYLF-GRYLRVQNSA-N
Smiles	N[C@@H](C(N/N=C/C3=CC=C(N=CC=N4)C4=C3)=O)CC2=CNC1=CC=CC=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Rhosin hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	7.9	20 with gentle warming
	DMSO	39.49	100

Preparing Stock Solutions for Rhosin hydrochloride

The following data is based on the product molecular weight 394.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.53 mL	12.66 mL	25.33 mL
5 mM	0.51 mL	2.53 mL	5.07 mL
10 mM	0.25 mL	1.27 mL	2.53 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

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References for Rhosin hydrochloride

References are publications that support the biological activity of the product.

Shang Rational design of small molecule inhibitors targeting RhoA subfamily Rho GTPases. Chem.Biol. 2012 PMID: 22726684
Shang Small-molecule inhibitors targeting G-protein-coupled Rho guanine nucleotide exchange factors. Proc.Natl.Acad.Sci.U.S.A. 2013 PMID: 23382194