Pyrimethamine

Name: Pyrimethamine
Brand: Tocris Bioscience
SKU: 3918

Catalog # 3918 | Tocris Bioscience a Bio-Techne Brand

Citations (1)

COA / SDS

Potent inhibitor of multidrug and toxin extrusion (MATE) transporters; also DHFR and STAT3 inhibitor

Key Product Details

Description:	Potent inhibitor of multidrug and toxin extrusion (MATE) transporters; also DHFR and STAT3 inhibitor
Chemical Name:	5-(4-Chlorophenyl)-6-ethyl-2,4-pyrimidinediamine
Purity:	≥99% (HPLC)
Molecular Weight:	248.71

View Full Technical Details for Pyrimethamine

Technical Data
Citations (1) / Reviews

Biological Activity for Pyrimethamine

Pyrimethamine is a potent inhibitor of multidrug and toxin extrusion (MATE) transporters (K_i values are 46 and 77 nM for human MATE2-K-HEK293 and MATE1-HEK293 cells respectively). Also inhibits dihydrofolate reductase (DHFR) and STAT3. Decreases proliferation of human autosomal dominant polycystic kidney disease cells.

Compound Libraries for Pyrimethamine

Pyrimethamine is also offered as part of the Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Pyrimethamine

M. Wt	248.71
Formula	C₁₂H₁₃ClN₄
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	58-14-0
PubChem ID	4993
InChi Key	WKSAUQYGYAYLPV-UHFFFAOYSA-N
Smiles	ClC1=CC=C(C2=C(N)N=C(N)N=C2CC)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Pyrimethamine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	24.87	100
	ethanol	2.49	10

Preparing Stock Solutions for Pyrimethamine

The following data is based on the product molecular weight 248.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.02 mL	20.10 mL	40.21 mL
5 mM	0.80 mL	4.02 mL	8.04 mL
10 mM	0.40 mL	2.01 mL	4.02 mL
50 mM	0.08 mL	0.40 mL	0.80 mL

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Reconstitution Calculator

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References for Pyrimethamine

References are publications that support the biological activity of the product.

Takakura Pyrimethamine inhibits adult polycystic kidney disease by modulating STAT signaling pathways. Hum.Mol.Genet. 2011 PMID: 21821671
Ito Potent and specific inhibition of mMate1-mediated efflux of type I organic cations in the liver and kidney by pyrimeth. J.Pharmacol.Exp.Ther. 2010 PMID: 20065018
Zimmerman Competitive inhibition of folate absorption by dihydrofolate reductase inhibitors, trimeth. and pyrimeth. Am.J.Clin.Nutr. 1987 PMID: 3630970

Citations for Pyrimethamine (1)

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Citations are publications that use Bio-Techne products. Selected citations for Pyrimethamine include:

Hui et al. (2020) Simultaneous Control of Endogenous and User-Defined Genetic Pathways Using Unique ecDHFR Pharmacological Chaperones. Cell Chem Biol. 27:622-634.e6
PMID: 32330442

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