PX 12

Name: PX 12
Brand: Tocris Bioscience
SKU: 2954

Catalog # 2954 | Tocris Bioscience a Bio-Techne Brand

Citations (2)

COA / SDS

Thioredoxin-1 inhibitor

Key Product Details

Description:	Thioredoxin-1 inhibitor
Alternative Names:	IV-2
Chemical Name:	2-[(1-Methylpropyl)dithio]-1H-imidazole
Purity:	≥99% (HPLC)
Molecular Weight:	188.31

View Full Technical Details for PX 12

Technical Data
Citations (2) / Reviews

Biological Activity for PX 12

PX 12 is a thioredoxin-1 (Trx-1) inhibitor; irreversibly inactivates Trx-1 as a substrate for reduction by thioredoxin reductase (TR); competitively inhibits the reduction of Trx-1 by TR (K_i = 30.8 μM). Attenuates expression of HIF-1α, VEGF and iNOS (IC₅₀ values are 7.2, 10.4 and 18.1 μM respectively). Exhibits antitumor activity; directly inhibits tubulin in vitro and decreases tumor microvessel density in vivo.

Technical Data for PX 12

M. Wt	188.31
Formula	C₇H₁₂N₂S₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	141400-58-0
PubChem ID	219104
InChi Key	BPBPYQWMFCTCNG-UHFFFAOYSA-N
Smiles	CC(CC)SSC1=NC=CN1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PX 12

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	18.83	100

Preparing Stock Solutions for PX 12

The following data is based on the product molecular weight 188.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.31 mL	26.55 mL	53.10 mL
5 mM	1.06 mL	5.31 mL	10.62 mL
10 mM	0.53 mL	2.66 mL	5.31 mL
50 mM	0.11 mL	0.53 mL	1.06 mL

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References for PX 12

References are publications that support the biological activity of the product.

Jordan The thioredoxin-1 inhibitor 1-methylpropyl 2-imidazolyl disulfide (PX-12) decreases vascular permeability in tumor xenografts monitored by dynamic contrast enhanced magnetic resonance imaging. Clin.Cancer Res. 2005 PMID: 15701837
Welsh The thioredoxin redox inhibitors 1-methylpropyl 2-imidazolyl disulfide and pleurotin inhibit hypoxia-induced factor 1α and vascular endothelial growth factor formation. Mol.Cancer.Ther. 2003 PMID: 12657718
Kirkpatrick Mechanisms of inhibition of the thioredoxin growth factor system by antitumor 2-imidazolyl disulfides. Biochem.Pharmacol. 1998 PMID: 9605422

Citations for PX 12 (2)

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Citations are publications that use Bio-Techne products. Selected citations for PX 12 include:

Shin et al. (2013) PX-12-induced HeLa cell death is associated with oxidative stress and GSH depletion. Oncol Lett. 6:1804
PMID: 24260080
Zhu et al. (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. Cell Death Differ. 31:31
PMID: 22463935

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