preQ1-azide
Tocris Bioscience, part of Bio-Techne | Catalog # 7833
preQ1 with azide handle for click chemistry
Key Product Details
Description
preQ1 with azide handle for click chemistry
Product Description
preQ1-azide is a modified analog of the natural substrate prequeosine1 (preQ1). It contains an azide handle allowing attachment of additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. preQ1-azide is a toolbox compound for RNA-TAG (transglycosylation at guanosine).Other RNA-TAG toolbox compounds include preQ1-biotin (Cat. No. 7804) and preQ1- alkyne (Cat. No. 7806)
Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.
Licensing Information
Sold under license from The Regents of the University of California.Product Specifications
Molecular Weight
419.49
Formula
C18H29N9O3
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-[6-[[(2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]amino]hexyl]-3-(2-azidoethoxy)propanamide
InChI Key
YYJIXWDJSKVSGF-UHFFFAOYSA-N
SMILES
O=C1C2=C(NC=C2CNCCCCCCNC(CCOCCN=[N+]=[N-])=O)N=C(N)N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.95 | 100 | |
| Ethanol | 20.97 | 50 |
Preparing Stock Solutions
for preQ1-azide
The following data is based on the product molecular weight 419.49
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.38 mL | 11.92 mL | 23.84 mL |
| 5 mM | 0.48 mL | 2.38 mL | 4.77 mL |
| 10 mM | 0.24 mL | 1.19 mL | 2.38 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhang Site-specific and enzymatic cross-linking of sgRNA enables wavelength-selectable photoactivated control of CRISPR gene editing. J.Am.Chem.Soc. 2022 PMID: 35257575
Product Specific Notices for preQ1-azide
For research use only
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