PR 619

Name: PR 619
Brand: Tocris Bioscience
SKU: 4482
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4482

(1)

Citations (14)

COA / SDS

Broad spectrum DUB inhibitor

Key Product Details

Description:	Broad spectrum DUB inhibitor
Chemical Name:	2,6-Diamino-3,5-dithiocyanopyridine
Purity:	≥98% (HPLC)
Molecular Weight:	223.28

View Full Technical Details for PR 619

Technical Data
Citations / Reviews (1)

Biological Activity for PR 619

PR 619 is a broad spectrum, reversible DUB inhibitor. Exhibits limited activity against other proteases. Induces accumulation of polyubiquitinated proteins, but has no direct inhibitory effect on the proteasome. Cytotoxic in HEK293T and colorectal cancer cells. Stabilizes microtubule network in oligodendroglial cells.

Technical Data for PR 619

M. Wt	223.28
Formula	C₇H₅N₅S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2645-32-1
PubChem ID	2817763
InChi Key	ZXOBLNBVNROVLC-UHFFFAOYSA-N
Smiles	NC1=C(SC#N)C=C(SC#N)C(N)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PR 619

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.23	10

Preparing Stock Solutions for PR 619

The following data is based on the product molecular weight 223.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	44.79 mL	223.93 mL	447.87 mL
0.5 mM	8.96 mL	44.79 mL	89.57 mL
1 mM	4.48 mL	22.39 mL	44.79 mL
5 mM	0.90 mL	4.48 mL	8.96 mL

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Reconstitution Calculator

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References for PR 619

References are publications that support the biological activity of the product.

Seiberlich The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases. Biochim.Biophys.Acta 2012 PMID: 22565157
Altun Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem.Biol. 2011 PMID: 22118674