Polybrene

Name: Polybrene
Brand: Tocris Bioscience
SKU: 7711
Rating: 0 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 7711

(1)

Citations (20)

COA / SDS

Viral transduction enhancer

Key Product Details

Description:	Viral transduction enhancer
Alternative Names:	Hexadimethrine bromide
Chemical Name:	1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
Purity:	≥95%

View Full Technical Details for Polybrene

Technical Data
Citations / Reviews (1)

Biological Activity for Polybrene

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols. It is commonly used in combination with Protamine sulfate (Cat. No. 8822) to enhance the delivery of adeno-associated virus (AAV) and lentiviral vectors.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

Technical Data for Polybrene

Storage	Store at +4°C
Purity	≥95%
CAS Number	28728-55-4
Smiles	C[N+](C)(CCC[N+](C)(CCCCCCC)C)C.[Br-].[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Polybrene

Solubility

Soluble to 100mg/ml in water

Dilution Calculator

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Reconstitution Calculator

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References for Polybrene

References are publications that support the biological activity of the product.

Elegheert Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 2018 PMID: 30455477
Kumar Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2021 PMID: 34746857
Joung CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 2022 PMID: 35338135
Navarro-Guerrero Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 2021 PMID: 33608581
Strack Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 2022 PMID: 35062296
Zhao Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 2014 PMID: 24658746
Bolis Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 2021 PMID: 34857732
Shayakhmetov Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 2000 PMID: 11044071
Abe Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 PMID: 9797877
Davis Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 2002 PMID: 12050007

Polybrene

Tocris Bioscience, part of Bio-Techne | Catalog # 7711

Key Product Details

Biological Activity for Polybrene

Technical Data for Polybrene

Solubility for Polybrene

Dilution Calculator

Reconstitution Calculator

References for Polybrene

Product Documents for Polybrene

COA

Certificate of Analysis

SDS

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