Polybrene

Product Description

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols. It is commonly used in combination with Protamine sulfate (Cat. No. 8822) to enhance the delivery of adeno-associated virus (AAV) and lentiviral vectors.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

Solubility

Calculators

Background References

References are publications that support the biological activity of the product.

• Elegheert Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 2018 PMID: 30455477
• Kumar Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2021 PMID: 34746857
• Joung CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 2022 PMID: 35338135
• Navarro-Guerrero Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 2021 PMID: 33608581
• Strack Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 2022 PMID: 35062296
• Zhao Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 2014 PMID: 24658746
• Bolis Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 2021 PMID: 34857732
• Shayakhmetov Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 2000 PMID: 11044071
• Abe Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 PMID: 9797877
• Davis Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 2002 PMID: 12050007