PMSF

Name: PMSF
Brand: Tocris Bioscience
SKU: 4486
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4486

(1)

Citations (22)

COA / SDS

Non-selective protease inhibitor; also blocks anandamide metabolism

Key Product Details

Description:	Non-selective protease inhibitor; also blocks anandamide metabolism
Chemical Name:	Phenylmethylsulfonyl fluoride
Purity:	≥98%
Molecular Weight:	174.19

View Full Technical Details for PMSF

Technical Data
Citations / Reviews (1)

Biological Activity for PMSF

PMSF is a non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t_1/2 ~ 60 mins at pH 7.5). Brain penetrant.

Technical Data for PMSF

M. Wt	174.19
Formula	C₇H₇FO₂S
Storage	Desiccate at RT
Purity	≥98%
CAS Number	329-98-6
PubChem ID	4784
InChi Key	YBYRMVIVWMBXKQ-UHFFFAOYSA-N
Smiles	O=S(CC1=CC=CC=C1)(F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PMSF

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	17.42	100
	DMSO	17.42	100

Preparing Stock Solutions for PMSF

The following data is based on the product molecular weight 174.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.74 mL	28.70 mL	57.41 mL
5 mM	1.15 mL	5.74 mL	11.48 mL
10 mM	0.57 mL	2.87 mL	5.74 mL
50 mM	0.11 mL	0.57 mL	1.15 mL

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Reconstitution Calculator

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References for PMSF

References are publications that support the biological activity of the product.

Compton & Martin The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J.Pharmacol.Exp.Ther. 1997 PMID: 9399986
Day Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide. Mol.Pharmacol. 2001 PMID: 11353795
Turini The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin. J.Pharmacol.Exp.Ther. 1969 PMID: 4306345