Picrotoxin stock was prepared in DMSO and diluted with ACSF to 25 micromolar concentration. Bath application of Picrotoxin blocked evoked inhibitory postsynaptic currents in an ex-vivo slice preparation.
Picrotoxin blocks single-channel openings of GABA-A receptor
20 microM Picrotoxin concentration was used to block GABA-A receptor openings in nucleated cell membrane patches. Full block was achieved in ~30 seconds after application. Picture: GABA-A receptor openings under control conditions (top three traces) and after application of Picrotoxin (bottom trace).
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