PHT 427

Name: PHT 427
Brand: Tocris Bioscience
SKU: 4598

Tocris Bioscience a Bio-Techne Brand

Catalog # 4598

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Key Product Details

Description:	Dual Akt and PDK1 inhibitor; antitumor
Chemical Name:	4-Dodecyl-N-1,3,4-thiadiazol-2-yl-benzenesulfonamide
Purity:	≥98% (HPLC)
Molecular Weight:	409.61

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Biological Activity for PHT 427

PHT 427 is a dual Akt and PDK1 inhibitor; binds the pleckstrin homology (PH) domain of Akt and PDK1 (K_i values are 2.7 and 5.2 μM, respectively). Inhibits Akt phosphorylation and Akt downstream targets; also induces apoptosis in vitro. Displays synergistic antitumor effect when combined with chemotherapeutics in breast cancer mouse models.

Technical Data for PHT 427

M. Wt	409.61
Formula	C₂₀H₃₁N₃O₂S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1191951-57-1
PubChem ID	44240850
InChi Key	BYWWNRBKPCPJMG-UHFFFAOYSA-N
Smiles	O=S(C(C=C1)=CC=C1CCCCCCCCCCCC)(NC2=NN=CS2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PHT 427

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.96	100

Preparing Stock Solutions for PHT 427

The following data is based on the product molecular weight 409.61.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.44 mL	12.21 mL	24.41 mL
5 mM	0.49 mL	2.44 mL	4.88 mL
10 mM	0.24 mL	1.22 mL	2.44 mL
50 mM	0.05 mL	0.24 mL	0.49 mL

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References for PHT 427

References are publications that support the biological activity of the product.

Meuillet Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor. Mol.Cancer Ther. 2010 PMID: 20197390
Moses In vitro and in vivo activity of novel small-molecule inhibitors targeting the pleckstrin homology domain of protein kinase B/AKT. Cancer Res. 2009 PMID: 19491272

Citations for PHT 427

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