Phorbol 12,13-dibutyrate

Name: Phorbol 12,13-dibutyrate
Brand: Tocris Bioscience
SKU: 4153
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 4153

Citations (3)

(1)

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Key Product Details

Description:	Protein kinase C activator
Alternative Names:	PDBu
Chemical Name:	(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity:	≥97% (HPLC)
Molecular Weight:	504.61

View Full Technical Details for Phorbol 12,13-dibutyrate

Biological Activity for Phorbol 12,13-dibutyrate

Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca²⁺ concentration. Also inhibits the activity of Na⁺,K⁺ ATPase in OK cells.

For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for Phorbol 12,13-dibutyrate

M. Wt	504.61
Formula	C₂₈H₄₀O₈
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	37558-16-0
PubChem ID	37783
InChi Key	BQJRUJTZSGYBEZ-YVQNUNKESA-N
Smiles	OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Phorbol 12,13-dibutyrate

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.23	50

Preparing Stock Solutions for Phorbol 12,13-dibutyrate

The following data is based on the product molecular weight 504.61.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.96 mL	19.82 mL	39.63 mL
2.5 mM	0.79 mL	3.96 mL	7.93 mL
5 mM	0.40 mL	1.98 mL	3.96 mL
25 mM	0.08 mL	0.40 mL	0.79 mL

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References for Phorbol 12,13-dibutyrate

References are publications that support the biological activity of the product.

Sakai Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 2010 PMID: 21187674
Hori Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 1999 PMID: 10460232
Middleton Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 1993 PMID: 8393456

Citations for Phorbol 12,13-dibutyrate (3)

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Citations are publications that use Bio-Techne products. Selected citations for Phorbol 12,13-dibutyrate include:

Nieuwenhuijze et al. (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol. 9:2234
PMID: 30323813
Nostro et al. (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol. 4:591
PMID: 25843049
Sánchez et al. (2014) Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors. Br J Pharmacol. 171:5682
PMID: 25091502

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PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes

Name: Chintan Koyani

Species: Other

Assay Type: In Vitro

Cell Line/Tissue: ventricular cardiomyocytes

Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.

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