PFI 7
Tocris Bioscience, part of Bio-Techne | Catalog # 7960
Selective GID4 antagonist
Key Product Details
Description
Selective GID4 antagonist
Product Description
PFI 7 is a selective GID4 antagonist (Kd = 80 nM by SPR assays and EC50 = 0.6 μM by cell-based NanoBRET). PFI 7 binds within the beta-barrel of GID4 substrate binding pocket, disrupting its interaction with the canonical Pro/N-degron peptide in live cells. It alters protein levels of several proteins including RNA helicases as measured by quantitative proteomics.Licensing Information
This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 7 probe summary on the SGC website.Product Specifications
Molecular Weight
474.43
Formula
C24H27N5O.2HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N-((1s,4s)-4-(1H-Benzo[d]imidazol-2-yl)cyclohexyl)-2-(((1H-indol-2-yl)methyl)amino)acetamide dihydrochloride
InChI Key
ZDMMYPOICXIDIM-QWLZZUQMSA-N
SMILES
O=C(CNCC1=CC2=CC=CC=C2N1)N[C@@H]3CC[C@@H](CC3)C4=NC5=CC=CC=C5N4.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 47.44 | 100 with gentle warming | |
| DMSO | 47.44 | 100 |
Preparing Stock Solutions
for PFI 7
The following data is based on the product molecular weight 474.43
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.11 mL | 10.54 mL | 21.08 mL |
| 5 mM | 0.42 mL | 2.11 mL | 4.22 mL |
| 10 mM | 0.21 mL | 1.05 mL | 2.11 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.42 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Owens A chemical probe to modulate human GID4 Pro/N-degron interactions. Nat.Chem.Biol. 2024 PMID: 38773330
Product Specific Notices for PFI 7
For research use only
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