PFI 7

Name: PFI 7
Brand: Tocris Bioscience
SKU: 7960

Tocris Bioscience, part of Bio-Techne | Catalog # 7960

COA / SDS

Selective GID4 antagonist

Key Product Details

Description:	Selective GID4 antagonist
Chemical Name:	N-((1s,4s)-4-(1H-Benzo[d]imidazol-2-yl)cyclohexyl)-2-(((1H-indol-2-yl)methyl)amino)acetamide dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	474.43

View Full Technical Details for PFI 7

Technical Data
Citations

Biological Activity for PFI 7

PFI 7 is a selective GID4 antagonist (K_d= 80 nM by SPR assays and EC₅₀= 0.6 μM by cell-based NanoBRET). PFI 7 binds within the beta -barrel of GID4 substrate binding pocket, disrupting its interaction with the canonical Pro/N-degron peptide in live cells. It alters protein levels of several proteins including RNA helicases as measured by quantitative proteomics.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 7 probe summary on the SGC website.

Technical Data for PFI 7

M. Wt	474.43
Formula	C₂₄H₂₇N₅O.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
InChi Key	ZDMMYPOICXIDIM-QWLZZUQMSA-N
Smiles	O=C(CNCC1=CC2=CC=CC=C2N1)N[C@@H]3CC[C@@H](CC3)C4=NC5=CC=CC=C5N4.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PFI 7

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	47.44	100 with gentle warming
	DMSO	47.44	100

Preparing Stock Solutions for PFI 7

The following data is based on the product molecular weight 474.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.11 mL	10.54 mL	21.08 mL
5 mM	0.42 mL	2.11 mL	4.22 mL
10 mM	0.21 mL	1.05 mL	2.11 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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References for PFI 7

References are publications that support the biological activity of the product.

Owens A chemical probe to modulate human GID4 Pro/N-degron interactions. Nat.Chem.Biol. 2024 PMID: 38773330