PEI STAR™ transfection reagent
Tocris Bioscience, part of Bio-Techne | Catalog # 7854
Key Product Details
| Description: | Polyethylenimine (PEI) transfection reagent, chemically-defined |
| Chemical Name: | Poly[imino(1,2-ethanediyl)] hydrochloride |
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Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance. |
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Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance. |
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Transfection of a reporter GFP construct using a leading competitor PEI and PEI STAR™. HEK293 cells grown in DMEM/10% FBS to 75% confluency in a 24 well plate. 1 μg of DNA + 25 μL optimem and 2 μg of PEI (1 mg/mL) + 25 μL optimem were incubated for 8 minutes before addition to cells. PEI STAR™ showed significantly higher expression of GFP in imaged cells. |