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Pam3CSK4, TLR1 and TLR2 Ligand

Catalog # NBP2-25297 | Novus Biologicals a Bio-Techne Brand
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NBP2-25297
NBP2-25297-0.025mg

Key Product Details

Species

Human, Mouse, Bacteria, Chicken

Applications

Functional Assay

Product Summary for Pam3CSK4, TLR1 and TLR2 Ligand

Pam3CSK4, TLR1 and TLR2 Ligand is a synthetic tripalmitoylated lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein. It activates monocytes and macrophages and is also a potent activator of proinflammatory transcription factor NF-kB. Pam3CSK4, TLR1 and TLR2 Ligand is recognized by a heterodimer formed between TLR1 and TLR2.Pam3CSK4, TLR1 and TLR2 Ligand stimulation: If your cell line does not naturally express TLR1+ TLR2, co-transfect with plasmids pCMV/TLR1and pCMV/TLR2. Forty-eight hrs after transfection, stimulate cells with 10 to 100 ng/ml of Pam3CSK4 for 6-24 hrs. Determine Pam3CSK4 stimulation using appropriate detection assays.

Product Specifications

Specificity

Pam3CSK4, TLR1 and TLR2 Ligand

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24105263). Mouse reactivity reported in scientific literature (PMID: 25511699) Use in Chicken reported in scientific publication (PMID: 32733155). Use in Bacteria reported in scientific publication (PMID: 31482074).

Scientific Data Images for Pam3CSK4, TLR1 and TLR2 Ligand

Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - 293 cells were transfected with pCMV/TLR1-2 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 ng/ml of Pam3CSK4 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit. pCMV/SEAP plasmid was used to check transfection efficiency.
Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Evaluation of the ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line. IML-102 cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase reporter gene under the transcriptional control of an NF-KB response element. IML-102 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of for 24 h. SEAP was analyzed using the SEAPorter Assay Kit . Data Summary: specifically activated the TLR2/1-depedent NF-KB/SEAP reporter cells in a dose dependent manner (Note: HEK 293 cells endogenously express TLR1).
Pam3CSK4, TLR1 and TLR2 Ligand [NBP2-25297] - Differentiated human macrophages were treated with or without 50 ng/mL Pam3CSK4 for 2 h, and the release of IL-6 in culture supernatants of cells was measured by ELISA kit.

Formulation, Preparation, and Storage

Formulation

Sterile Water

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pam3CSK4, TLR1 and TLR2 Ligand

Alternate Names

Pam3CSK4, Pam3CSK4 TLR1 and TLR2 Ligand, TLR1 and TLR2 Ligand

Product Documents for Pam3CSK4, TLR1 and TLR2 Ligand

Certificate of Analysis

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Product Specific Notices for Pam3CSK4, TLR1 and TLR2 Ligand

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for Pam3CSK4, TLR1 and TLR2 Ligand (30)

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  • Pam3CSK4, TLR1 and TLR2 Ligand
    Name: Eric Wang
    Verified Customer | Posted 06/21/2021
    Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297

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