Ouabain

Name: Ouabain
Brand: Tocris Bioscience
SKU: 1076

Catalog # 1076

Citations (9)

COA / SDS

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Key Product Details

Description:	Na⁺/K⁺ ATPase inhibitor
Chemical Name:	3-[(6-Deoxy-α-L-mannopyranosyl)oxy]-1,5,11α,14,19-pentahydoxycard-20(22)-enolide
Purity:	≥95% (HPLC)
Molecular Weight:	584.66

View Full Technical Details for Ouabain

Biological Activity

Ouabain is a selective Na⁺, K⁺-ATPase inhibitor. Cardiotonic agent. Also exhibits anti-MERS-CoV activity in Vero cells in vitro (IC₅₀ = 0.08 μM).

Technical Data

M. Wt	584.66
Formula	C₂₉H₄₄O₁₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	630-60-4
PubChem ID	439501
InChi Key	LPMXVESGRSUGHW-MJARETDLSA-N
Smiles	O[C@@]12[C@@]3([H])[C@]([C@]([C@H](O)C[C@H](O[C@H]4C(O)C(O)[C@@H](O)[C@H](C)O4)C5)(CO)[C@]5(O)CC3)([H])[C@H](O)C[C@]1(C)[C@@H](C(CO6)=CC6=O)CC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water		10
	DMSO	58.47	100

Preparing Stock Solutions

The following data is based on the product molecular weight 584.66.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.71 mL	8.55 mL	17.10 mL
5 mM	0.34 mL	1.71 mL	3.42 mL
10 mM	0.17 mL	0.86 mL	1.71 mL
50 mM	0.03 mL	0.17 mL	0.34 mL

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Reconstitution Calculator

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References

References are publications that support the biological activity of the product.

Ko Screening of FDA-approved drugs using a MERS-CoV clinical isolate from South Korea identifies potential therapeutic options for COVID-19. BioRxiv - Paper not yet peer reviewed
Croyle Extensive random mutagenesis analysis of the Na/K⁺ ATPase alpha subunit identifies known and previously unidentified amino acid residues that alter ouabain sensitivity-implications for ouabain binding. Eur.J.Biochem. 1997 PMID: 9346307
Hosoi Isoform-specific up-regulation by ouabain of Na⁺, K⁺-ATPase in cultured rat astrocytes. J.Neurochem. 1997 PMID: 9349566
Marjorie Ouabain improves cardiac function in vivo in rats with heart failure after chronic but not acute treatment. Naunyn Schmiedebergs Arch.Pharmacol. 1997 PMID: 9272726

Citations for Ouabain (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Ouabain include:

Babola et al. (2020) Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. Elife. 9:e52160
PMID: 31913121
Woo et al. (2019) Pharmacological Dissection of Intrinsic Optical Signal Reveals a Functional Coupling between Synaptic Activity and Astrocytic Volume Transient. Exp Neurobiol. 28:30
PMID: 30853822
Zhang et al. (2019) A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem. 2019;
PMID: 30792309
Romanos et al. (2019) Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation. Commun Biol. 2:127
PMID: 30963115
Yin et al. (2018) The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis. Nat Commun. 9:2815
PMID: 30022023
Ismael-Badarneh et al. (2015) The Role of Angiotensin II and Cyclic AMP in Alveolar Active Sodium Transport. PLoS One. 10:e0134175
PMID: 26230832
Pál et al. (2015) Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity. Nat Neurosci. 8:35
PMID: 26043770
Grassin-Delyle et al. (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res. 14:134
PMID: 24266887
Genda et al. (2011) Co-compartmentalization of the astroglial glutamate transporter, GLT-1, with glycolytic enzymes and mitochondria. J Neurosci. 31:18275
PMID: 22171032

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