O4I2

Name: O4I2
Brand: Tocris Bioscience
SKU: 5664

Tocris Bioscience a Bio-Techne Brand

Catalog # 5664

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Key Product Details

Description:	Oct3/4 inducer; induces expression of pluripotent-associated genes
Chemical Name:	Ethyl 2-[(4-chlorophenyl)amino]-4-thiazolecarboxylate
Purity:	≥98% (HPLC)
Molecular Weight:	282.75

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Biological Activity for O4I2

O4I2 is an Oct3/4 inducer; promotes Oct3/4 expression and stabilizes the Oct3/4 protein in HEK293 cells. Induces expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1. Induces 15-fold increase in Oct3/4 expression in human primary fibroblasts.

Compound Libraries for O4I2

O4I2 is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for O4I2

M. Wt	282.75
Formula	C₁₂H₁₁ClN₂O₂S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	165682-93-9
PubChem ID	53428670
InChi Key	ULUBAPWNHROTEU-UHFFFAOYSA-N
Smiles	ClC(C=C2)=CC=C2NC1=NC(C(OCC)=O)=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for O4I2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	28.27	100
	ethanol	14.14	50

Preparing Stock Solutions for O4I2

The following data is based on the product molecular weight 282.75.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.54 mL	17.68 mL	35.37 mL
5 mM	0.71 mL	3.54 mL	7.07 mL
10 mM	0.35 mL	1.77 mL	3.54 mL
50 mM	0.07 mL	0.35 mL	0.71 mL

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References for O4I2

References are publications that support the biological activity of the product.

Cheng Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and derivatives are potent inducers of Oct3/4. J.Med.Chem. 2015 PMID: 26143659

Citations for O4I2

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