NPPB

Tocris Bioscience a Bio-Techne Brand

Catalog # 0593

Citations (10)

(1)

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Key Product Details

Description:	Inhibits calcium-sensitive chloride currents
Chemical Name:	5-Nitro-2-(3-phenylpropylamino)benzoic acid
Purity:	≥99% (HPLC)
Molecular Weight:	300.31

View Full Technical Details for NPPB

Biological Activity for NPPB

Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.

Compound Libraries for NPPB

NPPB is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for NPPB

M. Wt	300.31
Formula	C₁₆H₁₆N₂O₄
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	107254-86-4
PubChem ID	4549
InChi Key	WBSMIPAMAXNXFS-UHFFFAOYSA-N
Smiles	OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NPPB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	30.03	100
	ethanol	6.01	20

Preparing Stock Solutions for NPPB

The following data is based on the product molecular weight 300.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.33 mL	16.65 mL	33.30 mL
5 mM	0.67 mL	3.33 mL	6.66 mL
10 mM	0.33 mL	1.66 mL	3.33 mL
50 mM	0.07 mL	0.33 mL	0.67 mL

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References for NPPB

References are publications that support the biological activity of the product.

Taniguchi 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 2008 PMID: 18818509
Kirkup Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 1996 PMID: 8825360
Keeling Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T₈₄ cell line. Biochim.Biophys.Acta 1991 PMID: 1720331
Wangemann Cl^--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 1986 PMID: 2434915

Citations for NPPB (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for NPPB include:

Rastogi et al. (2021) Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PLoS Pathog. 17:e1009712
PMID: 34324582
Morethson (2015) Extracellular fluid flow and chloride content modulate H(+) transport by osteoclasts. Cell Death Dis. 16:20
PMID: 26271334
Grant et al. (2013) Ionic currents of human trabecular meshwork cells from control and glaucoma subjects. Biol Proced Online. 246:167
PMID: 23135060
Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Membr Biol. 4:e969
PMID: 24357800
Sun et al. (2011) Lubiprostone reverses the inhibitory action of mor. on mucosal secretion in human small intestine. Dig Dis Sci. 56:330
PMID: 21181441
Sagheddu et al. (2010) Calcium concentration jumps reveal dynamic ion selectivity of calcium-activated chloride currents in mouse olfactory sensory neurons and TMEM16b-transfected HEK 293T cells. J Physiol. 588:4189
PMID: 20837642
Fei et al. (2009) Stimulation of mucosal secretion by lubipro. (SPI-0211) in guinea pig small intestine and colon. Am J Physiol Gastrointest Liver Physiol. 296:G823
PMID: 19179625
Silverman et al. (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol. 295:C761
PMID: 18596212
Li et al. (2005) Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin. Antimicrob Agents Chemother. 49:388
PMID: 15616319
Fioretti et al. (2005) Intermediate-conductance Ca2+-activated K+ channel is expressed in C2C12 myoblasts and is downregulated during myogenesis. Am J Physiol Cell Physiol. 289:C89
PMID: 15743891

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Customer Reviews for NPPB (1)

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A potent chloride channel blocker

Name: Anonymous

Species: Rat

Assay Type: Ex Vivo

It blocks calcium-activated chloride currents. We used NPPB at 50-100 microM to block chloride channels. It dissolves well in DMSO

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Product Documents for NPPB

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.