NPPB

Tocris Bioscience, part of Bio-Techne | Catalog # 0593

(1)

Citations (85)

COA / SDS

Inhibits calcium-sensitive chloride currents

Key Product Details

Description:	Inhibits calcium-sensitive chloride currents
Chemical Name:	5-Nitro-2-(3-phenylpropylamino)benzoic acid
Purity:	≥98% (HPLC)
Molecular Weight:	300.31

View Full Technical Details for NPPB

Technical Data
Citations / Reviews (1)

Biological Activity for NPPB

Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.

Compound Libraries for NPPB

NPPB is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for NPPB

M. Wt	300.31
Formula	C₁₆H₁₆N₂O₄
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	107254-86-4
PubChem ID	4549
InChi Key	WBSMIPAMAXNXFS-UHFFFAOYSA-N
Smiles	OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NPPB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	30.03	100
	ethanol	6.01	20

Preparing Stock Solutions for NPPB

The following data is based on the product molecular weight 300.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.33 mL	16.65 mL	33.30 mL
5 mM	0.67 mL	3.33 mL	6.66 mL
10 mM	0.33 mL	1.66 mL	3.33 mL
50 mM	0.07 mL	0.33 mL	0.67 mL

Molarity Calculator

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Dilution Calculator

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Reconstitution Calculator

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References for NPPB

References are publications that support the biological activity of the product.

Wangemann Cl^--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 1986 PMID: 2434915
Kirkup Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 1996 PMID: 8825360
Taniguchi 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 2008 PMID: 18818509
Keeling Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T₈₄ cell line. Biochim.Biophys.Acta 1991 PMID: 1720331