NPE-caged-proton is a 1-(2-nitrophenyl)ethyl caged proton that releases a proton and a sulfate ion upon photolysis at 350 - 355 nm. Generates rapid acidifications (pH jumps) down to pH 2.

Product Specifications

Molecular Weight

269.21

Formula

C₈H₈NNaO₆S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

1-(2-Nitrophenyl)ethyl sulfate sodium salt

CAS Number

1186195-63-0

PubChem ID

57369362

InChI Key

LCCXAXUWDTVOHF-UHFFFAOYSA-M

SMILES

[O-][N+](C1=C(C(C)OS([O-])(=O)=O)C=CC=C1)=O.[Na+]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	26.92	100
	DMSO	26.92	100

Preparing Stock Solutions for NPE-caged-proton

The following data is based on the product molecular weight 269.21

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.71 mL	18.57 mL	37.15 mL
5 mM	0.74 mL	3.71 mL	7.43 mL
10 mM	0.37 mL	1.86 mL	3.71 mL
50 mM	0.07 mL	0.37 mL	0.74 mL

Calculators

Molarity Calculator

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Mass

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g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

Background References

References are publications that support the biological activity of the product.

Abbruzzetti Kinetics of proton release after flash photolysis of 1-(2-Nitrophenyl)ethyl sulfate (caged sulfate) in aqueous solution. J.Am.Chem.Soc. 2005 PMID: 15998092
Peralvarez-Marin Time-resolved infrared spectroscopy of pH-induced aggregation of the Alzheimer Aβ_1-28 peptide. J.Mol.Biol. 2008 PMID: 18462754
Barth and Corrie Characterization of a new caged proton capable of inducing large pH jumps. Biophys.J. 2002 PMID: 12414718

NPE-caged-proton

Tocris Bioscience, part of Bio-Techne | Catalog # 3512

Key Product Details

Description

Alternative Names

View all Caged Compounds »

Product Description