Nocodazole

Name: Nocodazole
Brand: Tocris Bioscience
SKU: 1228
Rating: 5 (1 reviews)

Catalog # 1228 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (12)

COA / SDS

Microtubule inhibitor

Key Product Details

Description:	Microtubule inhibitor
Chemical Name:	[5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]carbonic acid, methyl ester
Purity:	≥95% (HPLC)
Molecular Weight:	301.32

View Full Technical Details for Nocodazole

Technical Data
Citations (12) / Reviews (1)

Biological Activity for Nocodazole

Nocodazole is a microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.

Technical Data for Nocodazole

M. Wt	301.32
Formula	C₁₄H₁₁N₃O₃S
Storage	Store at RT
Purity	≥95% (HPLC)
CAS Number	31430-18-9
PubChem ID	4122
InChi Key	KYRVNWMVYQXFEU-UHFFFAOYSA-N
Smiles	COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nocodazole

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	15.07	50

Preparing Stock Solutions for Nocodazole

The following data is based on the product molecular weight 301.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	6.64 mL	33.19 mL	66.37 mL
2.5 mM	1.33 mL	6.64 mL	13.27 mL
5 mM	0.66 mL	3.32 mL	6.64 mL
25 mM	0.13 mL	0.66 mL	1.33 mL

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Reconstitution Calculator

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References for Nocodazole

References are publications that support the biological activity of the product.

Lin Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 2014 PMID: 25497837
Mizushima Methods in mammalian autophagy research. Cell 2010 PMID: 20144757
Webb Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 2004 PMID: 15325591
Vasquez Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 1997 PMID: 9201709

Citations for Nocodazole (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Nocodazole include:

Paonessa et al. (2019) Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep. 26:582
PMID: 30650353
Bernier et al. (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep. 27:2895
PMID: 31167136
Macheret and Halazonetis (2018) Intragenic origins due to short G1 phases underlie oncogene-induced DNA replication stress. Nature. 555:112
PMID: 29466339
Romero et al. (2018) Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells. Nat Commun. 9:4010
PMID: 30275454
Guo et al. (2016) Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Front Cell Neurosci. 18:202
PMID: 26655835
Lin et al. (2015) Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Nat Commun. 6:18577
PMID: 26124177
Tauchman et al. (2015) Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Cell Biol. 6:10036
PMID: 26620470
Hutchins and Wray (2014) Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process. Methods Mol Biol. 8:400
PMID: 25505874
Melemedjian et al. (2014) Local translation and retrograde axonal transport of CREB regulates IL-6-induced nociceptive plasticity. Mol Pain. 10:45
PMID: 24993495
Seese et al. (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci. 32:7403
PMID: 22623686
Rood et al. (2011) Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Anal Biochem. 780:153
PMID: 21870260
Schapitz et al. (2010) Neuroligin 1 is dynamically exchanged at postsynaptic sites. Oncotarget. 30:12733
PMID: 20861378

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Customer Reviews for Nocodazole (1)

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Microtubule disruption

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Cell Line/Tissue: Brain slice

Used in brain slcies to disturb microtubule dynamics. Effect on cell shape was observed within minutes

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