NH 125

Product Description

NH 125 is an eukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC₅₀ = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC₅₀ values are 0.7 - 4.8 μM) and blocks G₁/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC₅₀ = 6.6 μM) and in vivo.

Solubility

Preparing Stock Solutions for NH 125

The following data is based on the product molecular weight 524.56

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.91 mL	9.53 mL	19.06 mL
5 mM	0.38 mL	1.91 mL	3.81 mL
10 mM	0.19 mL	0.95 mL	1.91 mL
50 mM	0.04 mL	0.19 mL	0.38 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Chen 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor. J.Biol.Chem. 2011 PMID: 22020937
• Arora Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines. Cancer Res. 2003 PMID: 14583488
• Arora P-glycoprotein mediates resistance to histidine kinase inhibitors. Mol.Pharmacol. 2004 PMID: 15322237
• Yamamoto Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci.Biotechnol.Biochem. 2001 PMID: 11758928