NH 125

Name: NH 125
Brand: Tocris Bioscience
SKU: 3439

Tocris Bioscience, part of Bio-Techne | Catalog # 3439

Citations (18)

COA / SDS

CaM kinase III (eEF-2 kinase) inhibitor

Key Product Details

Description:	CaM kinase III (eEF-2 kinase) inhibitor
Chemical Name:	1-Hexadecyl-2-methyl-3-(phenylmethyl)-1H-imidazolium iodide
Molecular Weight:	524.56

View Full Technical Details for NH 125

Technical Data
Citations

Biological Activity for NH 125

NH 125 is an eukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC₅₀ = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC₅₀ values are 0.7 - 4.8 μM) and blocks G₁/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC₅₀ = 6.6 μM) and in vivo.

Technical Data for NH 125

M. Wt	524.56
Formula	C₂₇H₄₅IN₂
Storage	Desiccate at -20°C
CAS Number	278603-08-0
PubChem ID	10436839
InChi Key	RVWOHCBHAGBLLT-UHFFFAOYSA-M
Smiles	CC1=[N+](CCCCCCCCCCCCCCCC)C=CN1CC2=CC=CC=C2.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NH 125

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	52.46	100
	ethanol	39.34	75

Preparing Stock Solutions for NH 125

The following data is based on the product molecular weight 524.56.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.91 mL	9.53 mL	19.06 mL
5 mM	0.38 mL	1.91 mL	3.81 mL
10 mM	0.19 mL	0.95 mL	1.91 mL
50 mM	0.04 mL	0.19 mL	0.38 mL

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References for NH 125

References are publications that support the biological activity of the product.

Chen 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor. J.Biol.Chem. 2011 PMID: 22020937
Arora Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines. Cancer Res. 2003 PMID: 14583488
Arora P-glycoprotein mediates resistance to histidine kinase inhibitors. Mol.Pharmacol. 2004 PMID: 15322237
Yamamoto Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci.Biotechnol.Biochem. 2001 PMID: 11758928