Necrostatin-1

Name: Necrostatin-1
Brand: Tocris Bioscience
SKU: 2324
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 2324

Citations (4)

(2)

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Key Product Details

Description:	RIP1 kinase inhibitor; inhibits necroptosis
Chemical Name:	5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity:	≥98% (HPLC)
Molecular Weight:	259.33

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Biological Activity for Necrostatin-1

Necrostatin-1 is an ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC₅₀ = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC₅₀ = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Compound Libraries for Necrostatin-1

Necrostatin-1 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for Necrostatin-1

M. Wt	259.33
Formula	C₁₃H₁₃N₃OS
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	4311-88-0
PubChem ID	2828334
InChi Key	TXUWMXQFNYDOEZ-UHFFFAOYSA-N
Smiles	CN1C(=S)NC(CC2=CNC3=CC=CC=C23)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Necrostatin-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.78	30

Preparing Stock Solutions for Necrostatin-1

The following data is based on the product molecular weight 259.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.3 mM	12.85 mL	64.27 mL	128.54 mL
1.5 mM	2.57 mL	12.85 mL	25.71 mL
3 mM	1.29 mL	6.43 mL	12.85 mL
15 mM	0.26 mL	1.29 mL	2.57 mL

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References for Necrostatin-1

References are publications that support the biological activity of the product.

Degterev Identification of RIP1 kinase as a specific cellular target of necroSTAT. Nat.Chem.Biol. 2008 PMID: 18408713
Degterev Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat.Chem.Biol. 2005 PMID: 16408008

Citations for Necrostatin-1 (4)

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Citations are publications that use Bio-Techne products. Selected citations for Necrostatin-1 include:

Chefetz et al. (2019) A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells. Cell Rep. 26:3061
PMID: 30865894
Greer et al. (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget. 9:18454
PMID: 29719618
Kim (2017) Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells Journal of Translational Medicine. 15(1):123
PMID: 28569199
Sagher et al. (2014) The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells. Cancer Cell Int. 14:75
PMID: 25324692

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Customer Reviews for Necrostatin-1 (2)

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Necroptosis-related studies

Name: MARINA KOUTSIOUMPA

Species: Human

Assay Type: In Vitro

Combinatorial treatments of Necrostatin-1 with other chemotherapeutic drugs were performed to elucidate the dependance of the latter on the induction of necroptosis. Results were consistent.
good necroptosis inhibitor

Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: Caco-2

100nM of Nec-1 was used to inhibit RIPK1 after administration cells with TNFa, zVADfmk, and smac inhibitors to induce necroptosis, then cell death rate was measured with a commercial kit.

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Product Documents for Necrostatin-1

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.