MPro N3

Name: MPro N3
Brand: Tocris Bioscience
SKU: 7230

Tocris Bioscience a Bio-Techne Brand

Catalog # 7230

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Coronavirus M^pro inhibitor
Chemical Name:	N-[(5-Methyl-3-isoxazolyl)carbonyl]-L-alanyl-L-valyl-N-[(1S,2E)-4-oxo-1-[[(3S)-2-oxo-3-pyrrolidinyl]methyl]-4-(phenylmethoxy)-2-buten-1-yl]-L-leucinamide
Purity:	≥98% (HPLC)
Molecular Weight:	680.8

View Full Technical Details for MPro N3

Biological Activity for MPro N3

MPro N3 is a coronavirus main protease (M^pro) inhibitor (respective IC₅₀ values are 2.7, 4 and 8.8 μM for MHV-A29, HCoV-229E and FOPV replication in vitro). Inhibits viral cell infection in an MHV plaque reduction assay. Also inhibits SARS-CoV-2 viral cell entry in a plaque reduction assay (IC₅₀ = 16.8 μM).

Compound Libraries for MPro N3

MPro N3 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for MPro N3

M. Wt	680.8
Formula	C₃₅H₄₈N₆O₈
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	884650-98-0
PubChem ID	6323191
InChi Key	IDBWWEGDLCFCTD-VNEMRZQUSA-N
Smiles	CC(C[C@@H](C(N[C@H](/C=C/C(OCC1=CC=CC=C1)=O)C[C@@H]2CCNC2=O)=O)NC([C@H](C(C)C)NC([C@@H](NC(C3=NOC(C)=C3)=O)C)=O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MPro N3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	34.04	50

Preparing Stock Solutions for MPro N3

The following data is based on the product molecular weight 680.8.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	2.94 mL	14.69 mL	29.38 mL
2.5 mM	0.59 mL	2.94 mL	5.88 mL
5 mM	0.29 mL	1.47 mL	2.94 mL
25 mM	0.06 mL	0.29 mL	0.59 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for MPro N3

References are publications that support the biological activity of the product.

Jin Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors. Nature. 2020 PMID: 32272481
Yang Design of wide-spectrum inhibitors targeting coronavirus main proteases. PLoS.One. 2005 PMID: 16128623

Citations for MPro N3

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.